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Quantitative analysis of hydrogen peroxide using ratiometric fluorescent probe-doped silica nanoparticles

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Abstract

Hydrogen peroxide (H2O2) is a representative indicator of various oxidative stresses. In this paper, we report a new silica nanoparticle platform using a combination of a multi-functional particle-based hybrid scaffold and ratiometric fluorescence probes, which can be self-calibrated for quantitative analysis. The chosen ratiometric fluorescent probe, Peroxy Lucifer 1 (PL1), was immobilized onto silica particles (PL1-SiO2) for the detection of cellular hydrogen peroxide by modulation of the internal charge transfer properties of the probe. In ratiometric analysis, a linear correlation between the hydrogen peroxide concentration and carbamate-derived emission intensities (F540/F470) was observed using PL1-SiO2. These silica particles doped with ratiometric fluorescent probes are a promising new scaffold for efficient determination of cellular hydrogen peroxide, which is a diagnostic biomarker of reactive oxygen metabolites.

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Author notes

  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Chemistry, Sahmyook University, Seoul, 01795, Republic of Korea

    Youngyoon Kim, Hyoung Sub Kim, Yooyeol Yang, Kyoungnae Baek & Myoung-Hwan Park

  2. Center for BioMicrosystems, Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul, 02792, Republic of Korea

    Hyoung Sub Kim & Nakwon Choi

Authors
  1. Youngyoon Kim
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  2. Hyoung Sub Kim
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  3. Yooyeol Yang
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  4. Kyoungnae Baek
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  5. Nakwon Choi
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Correspondence to Nakwon Choi or Myoung-Hwan Park.

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Kim, Y., Kim, H.S., Yang, Y. et al. Quantitative analysis of hydrogen peroxide using ratiometric fluorescent probe-doped silica nanoparticles. Toxicol. Environ. Health Sci. 9, 108–115 (2017). https://doi.org/10.1007/s13530-017-0310-8

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