Abstract
There have been studied on therapeutic of anticancer and antimicrobial using by effective proteins or peptides. In this study, we examined the lysosomal membrane protein extracted from lysosomes isolated from melanocyte treated by melanin with 100 ppm for 24 hours in order to find them reacted to targeting ability to melanin using by 2-dimensional electrophoresis (2DE). Consequently, we found 29 up-regulated (2.0 fold) and 23 down-regulated (2.0 fold) spots associated with melanin treatment. This result shown that lysosomal membrane proteins have function to react to treatment or decrease of melanin quantity through cellular activation of lysosomes.
This is a preview of subscription content, access via your institution.
References
Raposo, G. et al. Distinct Protein Sorting and Localization to Premelanosomes, Melanosomes, and Lysosomes in Pigmented Melanocytic Cells. J. Cell Biol. 152, 809–823 (2001).
Kricker, A., Armstrong, B. K. & English, D. R. Sun exposure and non-melanocytic skin cancer. Cancer Causes Control. 5, 367–392 (1994).
Yamaguchi, Y., Beer, J. Z. & Hearing, V. J. Melanin mediated apoptosis of epidermal cells damaged by ultraviolet radiation: factors influencing the incidence of skin cancer. Arch. Dermatol. Res. 300, 43–50 (2008).
Halaban, R. et al. Regulation of tyrosinase in human melanocytes grown in culture. J. Cell Biol. 97, 480–488 (1983).
Borges, C. R. Roberts, J. C. Wilkins, D. G. & Rollins, D. E. Relationship of melanin degradation products to actual melanin content: Application to human hair. Anal. Biochem. 290, 116–125 (2001).
Butler, M. J. Gardiner, R. B. & Day, A. W. Degradation of melanin or inhibition of its synthesis: are these a significant approach as a biological control of phytopathogenic fungi? Biol. Control. 32, 326–336 (2005).
No, J. K. et al. Inhibition of tyrosinase by green tea components. Life Sci. 65, 241–246 (1999).
Chan, E. W. C. et al. Antioxidant and tyrosinase inhibition properties of leaves and rhizomes of ginger species. Food Chem. 109, 477–483 (2008).
Bang, S. H. et al. Impact of colvent pH on direct immobilization of lysosome-related cell organelle extracts on TiO2 for melanin treatment. J. Microbiol. Biotechnol. 25, 718–722 (2015).
Park, D. J. et al. Different Expression Patterns of Lysosomal Proteins Exposed to Melanin in HeLa Cells. Toxcol. Environ. Health. Sci. 7, 1–5 (2015).
Yoon, J. et al. Melanin reduction by peroxidase activity in lysosome-related organelle extracts from hen egg whites, HeLa cells, and Saccharomyces cerevisiae. Mol. Cell. Toxicol. 11, 441–447 (2015).
Nhtaki, N. & Seiji, M. Degradation of Melanosomes by Lysosomes. J. Invest. Dermatol. 57, 1–5 (1971).
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Bang, S.H., Park, D.J., Kim, YH. et al. Lysosomal membrane proteins exposed to melanin in melanocyte B16F10. Toxicol. Environ. Health Sci. 8, 253–257 (2016). https://doi.org/10.1007/s13530-016-0282-0
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13530-016-0282-0