A high quantity of melanin causes hyperpigmentation or skin cancer in humans and it can also activate lysosomal enzymes much melanin. In this study, lysosomal proteins were identified through two-dimensional polyacrylamide gel electrophoresis (2-DE) after melanocytes were exposed to melanin (B16F10). Melanocytes were exposed to 100 ppm melanin for 24 hours and the lysosomal proteins were extracted. The results showed that 18 protein spots up regulated and 6 protein spots down regulated after melanin exposure. The expression pattern of established lysosomal proteins is informative in understanding proteins related to decreasing melanin.
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These authors contributed equally to this work.
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Park, D.J., Sekhon, S.S., Ahn, JY. et al. Proteomic analysis of lysosomal proteins in melanocyte B16F10 exposed to melanin. Toxicol. Environ. Health Sci. 8, 7–11 (2016). https://doi.org/10.1007/s13530-016-0256-2