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Proteomic analysis of lysosomal proteins in melanocyte B16F10 exposed to melanin

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A high quantity of melanin causes hyperpigmentation or skin cancer in humans and it can also activate lysosomal enzymes much melanin. In this study, lysosomal proteins were identified through two-dimensional polyacrylamide gel electrophoresis (2-DE) after melanocytes were exposed to melanin (B16F10). Melanocytes were exposed to 100 ppm melanin for 24 hours and the lysosomal proteins were extracted. The results showed that 18 protein spots up regulated and 6 protein spots down regulated after melanin exposure. The expression pattern of established lysosomal proteins is informative in understanding proteins related to decreasing melanin.

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  1. Andrzej, S., Desmond, J. T., Shigeki, S. & Jacobo, W. Melanin pigmentation in mammalian skin and its hormonal regulation. Physiol. Rev. 84, 1155–1228 (2004).

    Article  Google Scholar 

  2. Hans, C. W., Jane, S.-M., Takasi, K. & Robert, G. Skin aging and natural photo protection. Micron. 35, 185–191 (2004).

    Article  Google Scholar 

  3. Hideaki, F. et al. Evidence for distinct membrane traffic pathways to melanosomes and lysosomes in melanocytes. J. Invest. Dermatol. 6, 19–24 (2001).

    Article  Google Scholar 

  4. Michael, S. M. & Miguel, C. S. The melanosome: membrane dynamics in black and white. Molecul. Cell Biol. 2, 738–748 (2001).

    Google Scholar 

  5. Marusa, H. C., Katarina, P., Ales, S. & Boris, T. Lysosomal pathways to cell death and their therapeutic applications. Experimental Cell Res. 318, 1245–1251 (2012).

    Article  Google Scholar 

  6. Tardy, C. et al. Lysosomes and lysosomal proteins in cancer cell death. Biochim. Biophsol. Acta. 1765, 101–125 (2006).

    CAS  Google Scholar 

  7. Dorovansky, J. & Elleder, M. Melanosome degradation. Pigment Cell Res. 16, 280–286 (2003).

    Article  Google Scholar 

  8. Klaus, W. Herbert Honigsmann. Are melanosome complexes lysosomes. J. Invest. Dermatol. 59, 170–176 (1972).

    Google Scholar 

  9. Pajak, S. et al. Melanin endocytosis by cultured mammalian cells: a model for melanin in a cellular environment. Experimental Cell Res. 149, 513–526 (1983).

    Article  CAS  Google Scholar 

  10. Ohtaki, N. & Seiji, M. Degradation of melanosomes by lysosomes. J. Invest. Dermatol. 57, 1–5 (1971).

    Article  CAS  Google Scholar 

  11. Katrin, K. et al. Identification of novel lysosomal metrix proteins by proteome analysis. Proteomics 5, 3966–3978 (2005).

    Article  Google Scholar 

  12. Raposo, G. et al. Distinct protein sorting and localization to premelanosomes, melanosomes and lysosomes in pigmented melanocytic cells. J. Cell Biol. 152, 809–823 (2001).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Bang, S. H. et al. Proteomic analysis of Daphnia magna exposed to caffeine, ibuprofen, aspirin and tetracycline. Toxcol. Environ. Health Sci. 7, 97–104 (2015).

    Article  Google Scholar 

  14. Nguyen, N.-T. et al. Toxic detection in mine water based on proteomic analysis of lysosomal enzymes in Saccharomyces cerevisiae. Environ. Health Toxicol. 29, e2014019 (2014).

    Article  PubMed  PubMed Central  Google Scholar 

  15. Bang, S. H. et al. Acute and chronic toxicity assessment and the gene expression of Dhb, Vtg, Arnt, CYP4, and CYP314 in Daphnia magna exposed to pharmaceuticals. Mol. Cell. Toxicol. 11, 153–160 (2015).

    Article  CAS  Google Scholar 

  16. Bang, S. H. et al. Impact of colvent pH on direct immobilization of lysosome-related cell organelle extracts on TiO2 for melanin treatment. J. Microbiol. Biotechnol. 25, 718–722 (2015).

    Article  CAS  PubMed  Google Scholar 

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Correspondence to Yang-Hoon Kim or Jiho Min.

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These authors contributed equally to this work.

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Park, D.J., Sekhon, S.S., Ahn, JY. et al. Proteomic analysis of lysosomal proteins in melanocyte B16F10 exposed to melanin. Toxicol. Environ. Health Sci. 8, 7–11 (2016).

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