As a high quantity of melanin can lead to hyperpigmentation or skin cancer in humans and it can also activate lysosomal enzymes so in the present study the changes in lysosomal enzymes upon exposure of HeLa cells to melanin has been studied. The HeLa cells were exposed to 100 ppm melanin for 24 hours and lysosomal enzymes were extracted. The lysosomal enzymes were identified through twodimensional polyacrylamide gel electrophoresis (2DE) after exposure of HeLa cells to melanin. The results showed 12 up and 3down regulation spots in relation to melanin exposure. It has been observed that lysosomal proteins are related to melanin to decrease the color and quantity through cellular activation of lysosomes.
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These authors contributed equally to this work
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Park, D.J., Sekhon, S.S., Ahn, JY. et al. Different expression patterns of lysosomal proteins exposed to melanin in HeLa cells. Toxicol. Environ. Health Sci. 7, 272–276 (2015). https://doi.org/10.1007/s13530-015-0248-7