Skip to main content

Advertisement

SpringerLink
Log in

Tumor necrosis factor-alpha and high sensitivity C-reactive protein in young patients with diabetes mellitus

  • Original Article
  • Published:
International Journal of Diabetes in Developing Countries Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

Background

Inflammatory markers like tumor necrosis factor-α (TNF-α) and high-sensitivity C-reactive protein (hsCRP) have been thought to be associated with diabetes mellitus (DM). But their role in the development of diabetes in young is yet to be established.

Aims

To compare the concentrations of serum TNF-α and hsCRP between young subjects with normal glucose tolerance (NGT) and DM.

Methods

This cross-sectional study was performed with young subjects under 30 years of age consisting of 40 newly detected DM cases and an equal number of subjects with NGT diagnosed on the basis of the American Diabetes Association (ADA) criterion-2017 to see TNF-α and hsCRP levels. Glucose was measured by the glucose oxidase method, hsCRP by immunonephelometry, and TNF-α by enzyme-linked immunosorbent assay (ELISA).

Results

hsCRP was higher in DM than that of NGT [DM vs NGT: 3.41 (1.37–9.98) vs. 1.29 (0.68–2.65) pg/ml, median (IQR); p=0.003] but TNF-α was similar in both the groups (DM vs. NGT: 16.30±12.95 vs. 14.55±9.84 mg/L, mean±SD; p=0.201). Both body mass index (BMI) and waist circumference (WC) were positively correlated with hsCRP (BMI: r=0.501, p=0.001; WC: r=0.409, p=0.009) in NGT but not in DM (BMI: r=0.287, p=0.072; WC: r=0.281, p=0.078). Multiple regression analysis revealed BMI (p=0.020), WC (p=0.022), and hsCRP (p=0.041) as independent predictors for DM.

Conclusions

This study observed that serum hsCRP but not serum TNF-α was significantly higher in DM in young than that of NGT.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Arslanian S, Bacha F, Grey M, Marcus MD, White NH, Zeitler P. Evaluation and management of youth-onset type 2 diabetes: a positive statement by the American Diabetes Association. Diabetes Care. 2018;41(12):2648–68.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Schmidt MI, Duncan BB, Sharrett AR, Lindberg G, Savage PJ, Offenbacher S. Markers of Inflammation and prediction of diabetes mellitus in adults (Atherosclerosis Risk In Community study): a cohort study. Lancet. 1999;353:1649–52.

    Article  CAS  PubMed  Google Scholar 

  3. Duncan BB, Schmidt MI, Pankow JS, Ballantyne CM, Couper D, Vigo A, et al. Low-grade systemic inflammation and the development of type 2 diabetes: the atherosclerosis risk in communities study. Diabetes. 2003;52:1799–805.

    Article  CAS  PubMed  Google Scholar 

  4. Festa A, D’Agostino R Jr, Tracy RP, Haffner SM. Elevated levels of acute-phase proteins and plasminogen activator inhibitor-1 predict the development of type 2 diabetes. Diabetes. 2002;51:1131–7.

    Article  CAS  PubMed  Google Scholar 

  5. Pickup JC, Chusney GD, Thomas SM, Burt D. Plasma interleukin-6, tumour necrosis factor alpha and blood cytokine production in type 2 diabetes. Life Sci. 2000;67:291–300.

    Article  CAS  PubMed  Google Scholar 

  6. Akash MSH, Rehman K, Liaqat A. Tumor necrosis factor-alpha: role in development of insulin resistance and pathogenesis of type 2 diabetes mellitus. J Cell Biochem. 2018;119(1):105–10.

    Article  CAS  PubMed  Google Scholar 

  7. Spiegelman BM, Hotamisligil GS. Through thick and thin: wasting, obesity, and TNF alpha. Cell. 1993;73:625–7.

    Article  CAS  PubMed  Google Scholar 

  8. Hotamisligil GS, ShargillNS SBM. Adipose expression of tumor necrosis factor-alpha: direct role in obesity-linked insulin resistance. Science. 1993;259:87–91.

    Article  CAS  PubMed  Google Scholar 

  9. Mueller C, Held W, Imobden MA, Carnaud C. Accelerated beta-cell destruction in adoptively transferred autoimmune diabetes correlates with an increased expression of the genes coding for TNF-a and granzyme A in the intra-islet infiltrates. Diabetes. 1995;44:112–7.

    Article  CAS  PubMed  Google Scholar 

  10. Sproston NR, Ashworth JJ. Role of C-reactive protein at sites of inflammation and infection. Front Immunol. 2018;9:754.

    Article  PubMed  PubMed Central  Google Scholar 

  11. Wang A, Liu J, Li C, Gao J, Li X, Chen S, Wu S, Ding H, Fan H, Hou S. Cumulative exposure to high-sensitivity C-reactive protein predicts the risk of cardiovascular disease. J Am Heart Assoc. 2017;6(10):e005610.

    Article  PubMed  PubMed Central  Google Scholar 

  12. Pradhan AD, Manson JE, Rifai N, Buring JE, Ridker PM. C-reactive protein, interleukin 6, and risk of developing type 2 diabetes mellitus. JAMA. 2001;286(3):327–34.

    Article  CAS  PubMed  Google Scholar 

  13. Wolf M, Sandler L, Hsu K, Vossen-Smirnakis K, Ecker JL, Thadhani R. First-trimester C-reactive protein and subsequent gestational diabetes. Diabetes Care. 2003;26:819–24.

    Article  CAS  PubMed  Google Scholar 

  14. Tamakoshi K, Yatsuya H, Kondo T, Hori Y, Ishikawa M, Zhang H, Murata C, Otsuka R, Zhu S, Toyoshima H. The metabolic syndrome is associated with elevated circulating C-reactive protein in healthy reference range, and systemic low-grade inflammatory state. Int J Obes Relat Metab Disord. 2003;27:443–9.

    Article  CAS  PubMed  Google Scholar 

  15. Karavanaki K, Kakleas K, Georga S, Bartzeliotou A, Mavropoulos G, Tsouvalas M, Vogiatzi A, Papassotiriou I, Karayianni C. Plasma high sensitivity C-reactive protein and its relationship with cytokine levels in children with newly diagnosed type 1 diabetes and ketoacidosis. Clin Biochem. 2012;45(16-17):1383–8.

    Article  CAS  PubMed  Google Scholar 

  16. Wang X, Bao W, Liu J, Yang Y, Wang D, Rong S, et al. Inflammatory markers and risk of type 2 diabetes. Diabetes Care. 2013;36:166–75.

    Article  CAS  PubMed  Google Scholar 

  17. Dehghan A, Kardys I, de Maat MPM. Genetic variation, C-reactive protein levels, and incidence of diabetes. Diabetes. 2007;56:872–8.

    Article  CAS  PubMed  Google Scholar 

  18. Lee CC, Adler AI, Sandhu MS. Association of C-reactive protein with type 2 diabetes: prospective analysis and meta-analysis. Diabetologia. 2009;52:1040–7.

    Article  CAS  PubMed  Google Scholar 

  19. Konukoglu D, Hatemi H, Bayer H, Bagriacik N. Ralation between serum concentrations of interleukin-6 and tumor necrosis factor-𝛼 in female Turkish subjects with normal and impaired glucose tolerance. Horm Metab Res. 2006;38:34–7.

    Article  CAS  PubMed  Google Scholar 

  20. Choi KM, Lee J, Lee KW, Seo JA, Oh JH, Kim SG. Comparison of serum concentrations of C-reactive protein, TNF-𝛼 and IL-6 between elderly Korean women with normal and impaired glucose tolerance. Diabetes Res Clin Pract. 2004;64:99–106.

    Article  CAS  PubMed  Google Scholar 

  21. Hu FB, Meigs JB, Li TY, Rifai N, Manson JE. Inflammatory markers and risk of developing type 2 diabetes in women. Diabetes. 2004;53:693–700.

    Article  CAS  PubMed  Google Scholar 

  22. Hossain M, Faruque MO, Kabir G, Hassan N, Sikdar D, Nahar Q, Ali L. Association of serum TNF-a and IL-6 with insulin secretion and insulin resistance inIFG and IGT subjects in a Bangladeshi population. Int J Diabetes Mellit. 2010;2:165–8.

    Article  CAS  Google Scholar 

  23. Mirza S, Hossain M, Mathews C, Martinez P, Pino P, Gay JL, Rentfro A, McCormick JB, Fisher-Hoch SP. Type 2 diabetes is associated with elevated levels of TNF-alpha, IL-6 and adiponectin and low levels of leptin in a population of Mexican American: a cross-sectional study. Cytokine. 2012;57(1):136–42.

    Article  CAS  PubMed  Google Scholar 

  24. American Diabetes Association. Standards of medical care in diabetes-2019. Diabetes Care. 2019;42:13–28.

    Article  Google Scholar 

  25. Hannon TS, Rao G, Arslanian SA. Childhood obesity and type 2 diabetes mellitus. Pediatrics. 2005;116(2):473–80.

    Article  PubMed  Google Scholar 

  26. Copeland KC, Becker D, Gottschalk M, Hale D. Type 2 diabetes in children and adolescents: risk factors, diagnosis and treatment. Clinical Diabetes. 2005;23(4):181–5.

    Article  Google Scholar 

  27. Holt RIG, Cockram C, Flyyberg A, Goldstein BJ. editors. Textbook of Diabetes. 5th ed. West Sussex: Blackwell Publishing Ltd; 2017.

    Book  Google Scholar 

  28. Liu C, Feng X, Li Q, Wang Y, Li Q, Hua M. Adiponectin, TNF-α and inflammatory cytokines and risk of type 2 diabetes: a systematic review and meta-analysis. Cytokine. 2016;86:100–9.

    Article  CAS  PubMed  Google Scholar 

  29. Gupta A, Ten S, Anhalt H. Serum levels of soluble TNF-a receptor 2 are linkedto insulin resistance and glucose tolerance in children. Pediatric EndocrinolMetab. 2005;18:75–82.

    CAS  Google Scholar 

  30. Vinagre I, Sanchez-Quesada JL, Sanchez-Hernandez J, Santos D, Ordonez-Llanos J, Leiva AD, et al. Inflammatory biomarkers in type 2 diabetic patients: effect of glycemic control and impact of LDL subfraction phenotype. Cardiovasc Diabetol. 2014;13:34–9.

    Article  PubMed  PubMed Central  Google Scholar 

  31. Wang J, Yan R, Wen J, Kong X, Li H, Zhou P, Zhu H, Su X, Ma J. Association of lower body mass index with increased glycemic variability in patients with newly diagnosed type 2 diabetes: a cross-sectional study in China. Oncotarget. 2017;8(42):73133–43.

    Article  PubMed  PubMed Central  Google Scholar 

  32. Frank LK, Heraclides A, Danquah I, Bedu-Addo G, Mockenhaupt FP, Schulze MB. Measures of general and central obesity and risk of type 2 diabetes in a Ghanaian population. Trop Med Int Health. 2013;18(2):141–51.

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

The authors kindly acknowledge the contribution of the Department of Microbiology, BSMMU for their technical help and study on diabetes in young (SODY) group, BSMMU for their overall support.

Funding

This work was supported as research grant by the Research and Development, BSMMU and SODY group, BSMMU.

Author information

Authors and Affiliations

  1. Department of Endocrinology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Shahbagh, Dhaka, Bangladesh

    M. Imrul-Hasan,  Nusrat-Sultana,  Sharmin-Jahan,  Tahseen-Mahmood, M. Abu-Shehab & Muhammad Abul Hasanat

  2. Department of Endocrinology, Universal Medical College Hospital, Dhaka, Bangladesh

    Mohona-Zaman

  3. Department of Endocrinology, National Institute of Neurosciences & Hospital, Dhaka, Bangladesh

    Mashfiqul-Hasan

  4. Department of Microbiology & Immunology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Shahbagh, Dhaka, Bangladesh

    A. Abu-Saleh

Authors
  1. M. Imrul-Hasan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nusrat-Sultana
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sharmin-Jahan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tahseen-Mahmood
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. Abu-Shehab
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Mohona-Zaman
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Mashfiqul-Hasan
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. A. Abu-Saleh
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Muhammad Abul Hasanat
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Muhammad Abul Hasanat.

Ethics declarations

The research protocol was approved by the Institutional Review Board (IRB) of the institute. Informed written consent was obtained, and relevant clinical data were recorded from the participants.

Competing interest

The authors declare no competing interests.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Imrul-Hasan, M., Nusrat-Sultana, Sharmin-Jahan et al. Tumor necrosis factor-alpha and high sensitivity C-reactive protein in young patients with diabetes mellitus. Int J Diabetes Dev Ctries 43, 621–626 (2023). https://doi.org/10.1007/s13410-022-01124-w

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13410-022-01124-w

Keywords

Search

Navigation