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Association analysis of HHEX gene variant with type 2 diabetes risk

International Journal of Diabetes in Developing Countries volume 41, pages 43–47 (2021)Cite this article

Abstract

Background

Type 2 diabetes mellitus (T2DM) is a common, chronic, and complex disorder that is influenced by interactions between genetic and environmental factors. The hematopoietically expressed homeobox (HHEX) gene, which affects insulin sensitivity and secretion, is a candidate gene for the pathogenesis of T2DM.

Objective

The purpose of this study was to investigate the effect of the HHEX genetic variant, rs1111875, on the T2DM risk in a group of Iranian patients for the first time.

Methods

A total of 108 T2DM patients and 100 normal subjects were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).

Results

There was significant difference in genotypes and alleles frequency of rs1111875 between both case and control groups (p < 0.001). The frequency of the G allele was significantly higher in cases (87.5%) compared with controls (27%) (OR = 18.92, CI = 11.06–32.69, p < 0.001).

Conclusion

Our findings suggest that the rs1111875 variant of the HHEX gene could be considered a strong risk factor for T2DM development.

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Fig. 1

Data availability

Data from this project will be available to share.

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Acknowledgments

The researchers would like to express their gratitude to all participants enrolled in this project.

Author information

Authors and Affiliations

  1. Medical Genomics Research Center, Tehran Medical Sciences, Islamic Azad University, Khaghani Avenue, Shariati St, Tehran, 193951459, Iran

    Pantea Banihashemi & Mandana Hasanzad

  2. Personalized Medicine Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, No.10- Jalal -e-Ale-Ahmad Street, Chamran Highway, Tehran, 1411713119, Iran

    Hamid Reza Aghaei Meybodi & Negar Sarhangi

  3. Department of Community Medicine, Zabol University of Medical Sciences, Zabol, Iran

    Mahdi Afshari

Authors
  1. Pantea Banihashemi
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  2. Hamid Reza Aghaei Meybodi
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  3. Mahdi Afshari
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  4. Negar Sarhangi
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  5. Mandana Hasanzad
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Contributions

MH managed the project and provided guidance to the research; PB and NS drafted the manuscript and performed lab genotyping; MA performed the statistical analysis; HRAM provided clinical guidance to the research.

All authors contributed to and approved the final version of the manuscript.

Corresponding authors

Correspondence to Hamid Reza Aghaei Meybodi or Mandana Hasanzad.

Ethics declarations

Ethical approval and consent to participate

This study was approved by Ethical committee (ID research ethics: IR.IAU.PS.REC.1398.031) and informed consent was obtained from all individual participants included in the study.

Competing interests

The authors declare that they have no conflict of interest.

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Banihashemi, P., Aghaei Meybodi, H.R., Afshari, M. et al. Association analysis of HHEX gene variant with type 2 diabetes risk. Int J Diabetes Dev Ctries 41, 43–47 (2021). https://doi.org/10.1007/s13410-020-00870-z

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  • DOI: https://doi.org/10.1007/s13410-020-00870-z

Keywords

  • HHEX
  • rs1111875
  • T2DM
  • Genetic variant