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Association of adiponectin gene polymorphisms and their haplotypes with type 2 diabetes and related metabolic traits in an Iranian population

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International Journal of Diabetes in Developing Countries Aims and scope Submit manuscript

Abstract

Introduction

Adiponectin is an adipocyte-secreted protein that contributes to glucose homeostasis. Contradictory reports are available on single nucleotide polymorphisms (SNPs) in the adiponectin gene and the risk of type 2 diabetes (T2D). We investigate the association of adiponectin gene SNPs (+45T/G and +276G/T) with serum adiponectin, insulin resistance, lipid profile, and T2D risk in an Iranian population.

Method

The +45T/G and +276G/T SNPs were genotyped in 211 non-familial T2D patients and 202 non-diabetic subjects by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and TaqMan probe, respectively.

Results

T2D was associated with a decrease in serum adiponectin level. The G allele and the GG and TG genotypes of +45T/G SNP were more abundant than the T allele and the TT genotype in T2D patients compared with controls (p < 0.001). The risk of T2D in individuals with the GG and TG genotypes of +45T/G SNP was 4 and 2 times more than that with the TT genotype, respectively. There was no statistically significant difference in the frequencies of allele and genotype of +276G/T SNP between the control and T2D groups. The presence of +45G/+276G haplotype was associated with an increased risk of T2D (OR = 2.01, 95% CI = 1.34–3.03, p = 0.04).

Conclusion

Therefore, our results showed that +45T/G SNP is associated with the risk of T2D higher than +276G/T SNP in the studied population.

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Acknowledgments

We are most grateful to Vice Chancellor for Research Centers, Semnan University of Medical Sciences, for providing research facilities.

Funding

This work was supported by a grant (No. 271) from Semnan University of Medical Sciences and Golestan University of Medical Sciences.

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Authors and Affiliations

  1. Biochemistry & Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran

    Hamid Reza Joshaghani

  2. Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran

    Parviz Kokhaei & Mehdi Barati

  3. Nervous System Stem Cells Research Center, Semnan University of Medical Sciences, Semnan, Iran

    Abbas Pakdel

  4. Department of Biochemistry, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, 1513138111, Iran

    Abbas Pakdel

  5. Hyperlipidemia Research Center, Dept. of Clinical Biochemistry, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Ghorban Mohammadzadeh

  6. Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran

    Navid Bandegi

  7. Research Center of Physiology, Semnan University of Medical Sciences, Semnan, Iran

    Ahmad Reza Bandegi

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  1. Hamid Reza Joshaghani
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  2. Parviz Kokhaei
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Correspondence to Ahmad Reza Bandegi.

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The study has been approved by the appropriate local ethics committee at the Semnan University of Medical Sciences and has been performed in accordance with the ethical standards as laid down in the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the Semnan University of Medical Sciences research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Joshaghani, H.R., Kokhaei, P., Barati, M. et al. Association of adiponectin gene polymorphisms and their haplotypes with type 2 diabetes and related metabolic traits in an Iranian population. Int J Diabetes Dev Ctries 40, 216–222 (2020). https://doi.org/10.1007/s13410-019-00785-4

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  • DOI: https://doi.org/10.1007/s13410-019-00785-4

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