Skip to main content

Advertisement

SpringerLink
Log in

Serum 8-hydroxy-2′-deoxyguanosine (8-oxo-dG) levels are elevated in diabetes patients

  • Original Article
  • Published:
International Journal of Diabetes in Developing Countries Aims and scope Submit manuscript

Abstract

The increased oxidative stress in diabetes is known to contribute to the development of diabetes. We investigate whether serum 8-hydro-2′-deoxyguanosine (8-oxo-dG) is associated with diabetes at the time of first diagnosis and evaluate whether it can be used as a reliable biomarker for the oxidative stress in diabetes. The study was designed as a case control study with two groups: patient with diabetes and control. The diabetes group consisted of a total of 28 patients consulting the hospital for the first time and definitely diagnosed for diabetes, and the control group was composed of 65 healthy subjects. Serum 8-oxo-dG was measured by a competitive enzyme-linked immunosorbent assay (ELISA) kit, specially developed to minimize cross-reaction of 8-oxo-dG antibody with serum guanosine. The average serum 8-oxo-dG levels in patients with diabetes and controls were 0.72 ± 0.41 and 0.24 ± 0.14 ng/mL, respectively, statistically significant (p < 0.001). The 8-oxo-dG value was significantly higher in women with diabetes, compared with men with diabetes (p = 0.028). The sensitivity and the specificity of the 8-oxo-dG ELISA assay were 0.80 and 0.96, respectively, and the ROC value was 0.93. This study suggests that increased oxidative stress has an important role in the pathogenesis of diabetes. Serum 8-oxo-dG may be a useful clinical biomarker for the early diagnosis of stress-related diseases, e.g. diabetes and its management.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Broedbaem K, Weimann A, Stovgaard ES, et al. Urinary 8-oxo-7,8-dihydro-2’-deoxyguanosine as a biomarker in type 2 diabetes. Free Radical Biol Med. 2011;51(8):1473–9.

    Article  Google Scholar 

  2. Piconi L, Quagliaro L, Ceriello A. Oxidative stress in diabetes. Clin Chem Lab Med. 2003;41(9):1144–9.

    Article  CAS  PubMed  Google Scholar 

  3. Opara EC. Oxidative stress, micronutrients, diabetes mellitus and its complications. J R Soc Promot Health. 2002;122(1):28–34.

    Article  CAS  PubMed  Google Scholar 

  4. Nishikawa T, Edelstein D, Du XL, et al. Normalizing mitochondrial superoxide production blocks three pathways of hyperglycaemic damage. Nature. 2000;404(6779):787–90.

    Article  CAS  PubMed  Google Scholar 

  5. Loft S, Poulsen HE. Markers of oxidative damage to DNA: antioxidants and molecular damage. Methods Enzymol. 1999;300:166–84.

    Article  CAS  PubMed  Google Scholar 

  6. Dong QY, Cui Y, Chen L, et al. Urinary 8-hydroxydeoxyguanosine levels in diabetic retinopathy patients. Eur J Ophthalmol. 2008;18(1):94–8.

    CAS  PubMed  Google Scholar 

  7. Xu GW, Yao QH, Weng QF, et al. Study of urinary 8-hydroxydeoxyguanosine as a biomarker of oxidative DNA damage in diabetic nephropathy patients. J Pharm Biomed Anal. 2004;36(1):101–4.

    Article  CAS  PubMed  Google Scholar 

  8. Ha H, Kim C, Son Y, et al. DNA damage in the kidneys of diabetic rats exhibiting microalbuminuria. Free Radical Biol Med. 1994;16(2):271–4.

    Article  CAS  Google Scholar 

  9. Haghdoost S, Czene S, Naslund I, et al. Extracellular 8-oxo-dG as a sensitive parameter for oxidative stress in vivo and in vitro. Free Radical Res. 2005;39(2):153–62.

    Article  CAS  Google Scholar 

  10. Wu LL, Chiou CC, Chang PY, et al. Urinary 8-OHdG: a marker of oxidative stress to DNA and a risk factor for cancer, atherosclerosis and diabetics. Clin Chim Acta. 2004;339(1–2):1–9.

    Article  CAS  PubMed  Google Scholar 

  11. Broedbaek K, Siersma V, Henriksen T, et al. Urinary markers of nucleic acid oxidation and long-term mortality of newly diagnosed type 2 diabetic patients. Diabetes Care. 2011;34(12):2594–6.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  12. Sangsuwan T, Haghdoost S. The nucleotide pool, a target for low-dose gamma-ray-induced oxidative stress. Radiat Res. 2008;170(6):776–83.

    Article  CAS  PubMed  Google Scholar 

  13. Harms-Ringdahl M, Jenssen D, Haghdoost S. Tomato juice intake suppressed serum concentration of 8-oxodG after extensive physical activity. Nutr J. 2012;11:29.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  14. Haghdoost S, Sjolander L, Czene S, et al. The nucleotide pool is a significant target for oxidative stress. Free Radic Biol Med. 2006;41(4):620–6.

    Article  CAS  PubMed  Google Scholar 

  15. Mascarenhas-Melo F, Marado D, Palavra F, et al. Diabetes abrogates sex differences and aggravates cardiometabolic risk in postmenopausal women. Cardiovasc Diabetol. 2013;12:61.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  16. Hinokio Y, Suzuki S, Hirai M, et al. Urinary excretion of 8-oxo-7,8-dihydro-2′-deoxyguanosine as a predictor of the development of diabetic nephropathy. Diabetologia. 2002;45(6):877–82.

    Article  CAS  PubMed  Google Scholar 

  17. Bonnefont-Rousselot D. Glucose and reactive oxygen species. Curr Opin Clin Nutr Metab Care. 2002;5(5):561–8.

    Article  CAS  PubMed  Google Scholar 

  18. Evans JL, Goldfine ID, Maddux BA, et al. Oxidative stress and stress-activated signaling pathways: a unifying hypothesis of type 2 diabetes. Endocr Rev. 2002;23(5):599–622.

    Article  CAS  PubMed  Google Scholar 

  19. Maritim AC, Sanders RA, Watkins JB. Diabetes, oxidative stress, and antioxidants: a review. J Biochem Mol Toxicol. 2003;17(1):24–38.

    Article  CAS  PubMed  Google Scholar 

  20. Mohamed AK, Bierhaus A, Schiekofer S, et al. The role of oxidative stress and NF-kappaB activation in late diabetic complications. Biofactors. 1999;10(2–3):157–67.

    Article  CAS  PubMed  Google Scholar 

  21. Haghdoost S, Maruyama Y, Pecoits-Filho R, et al. Elevated serum 8-oxo-dG in hemodialysis patients: a marker of systemic inflammation? Antioxid Redox Signal. 2006;8(11–12):2169–73.

    Article  CAS  PubMed  Google Scholar 

  22. Nakabeppu Y. Molecular genetics and structural biology of human MutT homolog, MTH1. Mutat Res. 2001;477(1–2):59–70.

    Article  CAS  PubMed  Google Scholar 

  23. Boonla C, Wunsuwan R, Tungsanga K, et al. Urinary 8-hydroxydeoxyguanosine is elevated in patients with nephrolithiasis. Urol Res. 2007;35(4):185–91.

    Article  CAS  PubMed  Google Scholar 

  24. Kanauchi M, Nishioka H, Hashimoto T. Oxidative DNA damage and tubulointerstitial injury in diabetic nephropathy. Nephron. 2002;91(2):327–9.

    Article  CAS  PubMed  Google Scholar 

  25. Leinonen J, Lehtimaki T, Toyokuni S, et al. New biomarker evidence of oxidative DNA damage in patients with non-insulin-dependent diabetes mellitus. FEBS Lett. 1997;417(1):150–2.

    Article  CAS  PubMed  Google Scholar 

  26. Hinokio Y, Suzuki S, Hirai M, et al. Oxidative DNA damage in diabetes mellitus: its association with diabetic complications. Diabetologia. 1999;42(8):995–8.

    Article  CAS  PubMed  Google Scholar 

  27. ChiouCC CPY, Chan EC, et al. Urinary 8-hydroxydeoxyguanosine and its analogs as DNA marker of oxidative stress: development of an ELISA and measurement in both bladder and prostate cancers. Clin Chim Acta. 2003;334(1–2):87–94.

    Google Scholar 

  28. Al-Aubaidy HA, Jelinek HF. Oxidative DNA damage and obesity in type 2 diabetes mellitus. Eur J Endocrinol. 2011;164(6):899–904.

    Article  CAS  PubMed  Google Scholar 

  29. Topic A, Francuski D, Markovic B, et al. Gender-related reference intervals of urinary 8-oxo-7,8-dihydro-2’-deoxyguanosine determined by liquid chromatography-tandem mass spectrometry in Serbian population. Clin Biochem. 2013;46(4–5):321–6.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by the Shanghai Committee of Science and Technology Development Funds for Basic Research under grant number 10JC1404800. Health Biomarker Sweden AB also supported this study.

Conflict of interest

MHR, SH and SS are owner of Health Biomarker Sweden. All other authors declare no conflict of interest.

Author information

Author notes

    Authors and Affiliations

    1. Department of Endocrinology, Huadong Hospital, Fudan University, Shanghai, China

      Jiao Sun, Xudan Lou & Haidong Wang

    2. Department of Genetics, Microbiology and Toxicology, Stockholm University, Stockholm, Sweden

      Alice Sollazzo, Mats Harms-Ringdahl & Siamak Haghdoost

    3. Sino-Swed Molecular Bio-Medicine Research Institute, Shenzhen, China

      Sven Skog & Ellen He

    Authors
    1. Jiao Sun
      View author publications

      You can also search for this author in PubMed Google Scholar

    2. Xudan Lou
      View author publications

      You can also search for this author in PubMed Google Scholar

    3. Haidong Wang
      View author publications

      You can also search for this author in PubMed Google Scholar

    4. Alice Sollazzo
      View author publications

      You can also search for this author in PubMed Google Scholar

    5. Mats Harms-Ringdahl
      View author publications

      You can also search for this author in PubMed Google Scholar

    6. Sven Skog
      View author publications

      You can also search for this author in PubMed Google Scholar

    7. Ellen He
      View author publications

      You can also search for this author in PubMed Google Scholar

    8. Siamak Haghdoost
      View author publications

      You can also search for this author in PubMed Google Scholar

    Corresponding author

    Correspondence to Haidong Wang.

    Additional information

    Dr. Jiao Sun and Dr. Xudan Lou contribute to the work equally and should be regarded as co-first authors.

    Rights and permissions

    Reprints and permissions

    About this article

    Check for updates. Verify currency and authenticity via CrossMark

    Cite this article

    Sun, J., Lou, X., Wang, H. et al. Serum 8-hydroxy-2′-deoxyguanosine (8-oxo-dG) levels are elevated in diabetes patients. Int J Diabetes Dev Ctries 35, 368–373 (2015). https://doi.org/10.1007/s13410-015-0301-8

    Download citation

    • Received:

    • Accepted:

    • Published:

    • Issue Date:

    • DOI: https://doi.org/10.1007/s13410-015-0301-8

    Keywords

    Search

    Navigation