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Reactive oxygen species up-regulates SOCS-3 in 3T3-L1 adipocytes

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Abstract

We investigated the effect of increased intracellular reactive oxygen species(ROS) on SOCS-3 expression in 3T3-L1 adipocytes. Increased intracellular ROS levels in 3T3-L1 adipocytes were achieved by two methods of exposure to H2O2 and the occurrence of oxidative stress in cells was assessed by flow cytometry . Expression of SOCS-3 mRNA and that of some adipokines were measured by real time PCR. The level of SOCS-3 protein was determined by western blot. The effect of the antioxidant alpha-lipoic acid was also investigated. Both the relatively mild increased intracellular ROS and the acute but transient increased ROS elevated the levels of SOCS-3 mRNA and protein in 3T3-L1 adipocytes, acompanied with elevated levels of TNF-α mRNA and resistin mRNA and the decreased levels of adiponectin mRNA and secretory adiponectin in culture medium. α-lipoic acid could attenuate the effects of ROS on 3T3-L1 adipocytes. We hypothesized that in mature 3T3-L1 adipocytes, SOCS-3 could be upregulated directly by the induction of increased intracellular ROS and to some extent which was up-regulated by adipokine modulation.

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Acknowledgments

This study was supported by the Science and Technology’s Major Project of Zhejiang Province (Grant No. 2009C030104) . The National Key Technology R&D Program of China(Grant No. 2009BAI80B01) The Research Project of Education Bureau of Zhejiang Province (No. Y200908149). We thank Dr Li Ai-qing, Zhang Fu-biao,Shi Qing-lan and Tang Hai-mei (Key Laboratory of Biotherapy of Zhejiang Province, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine) for technical assistance and helpful discussions.

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  1. Department of Endocrinology of Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, 310016, People’s Republic of China

    Yu Ruan, Fen-ping Zheng, Lin Li, Zhou Wang & Hong Li

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  1. Yu Ruan
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  2. Fen-ping Zheng
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  3. Lin Li
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  4. Zhou Wang
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  5. Hong Li
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Correspondence to Hong Li.

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Ruan, Y., Zheng, Fp., Li, L. et al. Reactive oxygen species up-regulates SOCS-3 in 3T3-L1 adipocytes. Int J Diabetes Dev Ctries 35 (Suppl 3), 471–476 (2015). https://doi.org/10.1007/s13410-014-0201-3

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  • DOI: https://doi.org/10.1007/s13410-014-0201-3

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