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Development of gold, silver, and iron nanoparticles using isolated berberine: its characterization and antimicrobial and cytotoxic activities against COLO320DM and Hela cells

Abstract

The current report discloses the development of gold, silver, and iron nanoparticles using berberine by green synthesis and its evaluation for antimicrobial activity and in vitro cytotoxicity against colon cancer and human cervical cancer cells. The prepared nanoparticles were characterized by Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction analysis. The characterization of results of the prepared nanoparticles satisfied the distinctive properties of the potent nanodrug. The antimicrobial potential was exhibited by all the prepared nanoparticles against the selected microbial strains. The zone of inhibition (mm) exhibited by gold nanoparticles biosynthesized using berberine (BAuNPs) was 15.66 ± 0.47 mm against Pseudomonas aeruginosa, 15.0 ± 1.41 against Escherichia coli, and 15.33 ± 1.24 mm against Staphylococcus aureus. The cytotoxicity study exhibited promising results as compared to pure berberine. The results of the MTT assay of gold nanoparticles against COLO320DM exhibited 92.27 ± 0.14% inhibition, whereas in the case of the SRB assay, it revealed 89.72 ± 0.29% inhibition as compared to other nanoparticles and pure berberine. For Hela cell lines, in the case of the MTT assay, BAuNPs exhibited 92.13 ± 0.64% inhibition while in the SRB assay, it revealed 85.56 ± 0.39% inhibition. The results of the Trypan blue assay revealed that BAuNPs showed 76.54 ± 1.00% inhibition against the COLO320DM cell line, whereas in the case of Hela cell lines, 78.03 ± 1.47% inhibition was observed. The effect exhibited by BAuNPs may be attributed to increased production of reactive oxygen species by treated carcinoma cells that results in oxidation of vital constituents which ultimately causes death of the cell. Thus, these in vitro results demonstrate the promising therapeutic applications of the BAuNPs in nanomedicine.

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Acknowledgements

The authors are thankful to Hon. Secretary of KE Society Kasegaon and Principal of Rajarambapu College of Pharmacy, Kasegaon, Sangli, Maharashtra, for providing the facilities. We are also thankful to the managing director of Influx Healthcare, Mumbai, for providing pure phytoconstituent markers and Dr. Sandip Patil, MD (Biocyte Institute of Research and Development (BiRD), Sangli (MS).

Funding

The research work is supported under Research Grant to a college teacher by Shivaji University (SU), Kolhapur, MS, India, as an encouragement for the academic young scientist, and the sanction letter number is SU/C & UD Section UGK/&/1028 (date 29 Dec 2018).

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MAB made substantial contributions to collection and interpretation of data and to manuscript preparation/writing manuscript and performed the experiment. DSR, SDB, and KPS made substantial contributions to supervising experimental work. GHW and SST made substantial contributions to correction of the manuscript. All data were generated in-house, and no paper mill was used. All authors agree to be accountable for all aspects of work ensuring integrity and accuracy.

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Correspondence to Mangesh A. Bhutkar.

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Bhutkar, M.A., Randive, D.S., Bhinge, S.D. et al. Development of gold, silver, and iron nanoparticles using isolated berberine: its characterization and antimicrobial and cytotoxic activities against COLO320DM and Hela cells. Gold Bull 55, 93–103 (2022). https://doi.org/10.1007/s13404-022-00309-9

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  • DOI: https://doi.org/10.1007/s13404-022-00309-9

Keywords

  • Silver nanoparticles
  • Gold nanoparticles
  • Cytotoxicity
  • Phytoconstituents
  • Antimicrobial activity