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Synergic effect of paclitaxel and cisplatin associated with gold nanoparticles on HeLa cervical cells

Abstract  

The combination of two or more chemotherapy drugs and nanotechnology has emerged as an effective strategy in the fight against cancer. Paclitaxel (PTX) and cisplatin show improved cytotoxic effects on being used together in the chemotherapy of various cancer types, than when used individually. However, these chemotherapy drugs have been shown to have numerous toxic effects in cancer patients. Therefore, the aim of this study was to evaluate the cytotoxicity of PTX and cisplatin associated with gold nanoparticles (AuNps) on HeLa cells containing multiple copies of human papillomavirus 18. AuNps were successfully synthesized with a mean size of 20 to 61 nm, polydispersity index ≥ 0.3, and negative surface charge. Together, PTX and cisplatin associated with AuNps showed a higher cytotoxic effect on HeLa cells than free drugs. The combination of PTX and cisplatin with AuNps potentiated their cytotoxic effects, inducing synergism between the treatments, resulting in HeLa cell death by apoptosis.

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Funding

This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brazil (CAPES)—Finance Code 001, and by the Conselho Nacional de Pesquisa e Desenvolvimento—Brazil (CNPq) (404966/2018–7 and 311033/2019–9). Fundação do Amparo à Pesquisa e Inovação do Estado de Santa Catarina (FAPESC).

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Correspondence to Ricardo Andrez Machado-de-Ávila.

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Uggioni, M.L.R., Feuser, P.E., Possato, J.C. et al. Synergic effect of paclitaxel and cisplatin associated with gold nanoparticles on HeLa cervical cells. Gold Bull 55, 65–75 (2022). https://doi.org/10.1007/s13404-021-00305-5

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  • DOI: https://doi.org/10.1007/s13404-021-00305-5

Keywords

  • Cisplatin
  • Cytotoxicity
  • Gold nanoparticles
  • HeLa
  • Paclitaxel
  • Synergism