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In vivo detection demonstrates circulating tumor cell reduction instead of baseline number has prognostic value in bladder cancer patients receiving neoadjuvant chemotherapy

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Abstract

Purpose

Previous studies have suggested the potential prognostic value of circulating tumor cells (CTCs) in bladder cancer (BC) patients. This study aims to validate the prognostic value of in vivo detection of CTCs in muscle invasive bladder cancer (MIBC) patients receiving neoadjuvant chemotherapy (NAC).

Methods

A total of 107 MIBC patients were enrolled in this study. All patients had one in vivo detection of CTCs before initial treatment as baseline, and those who received neoadjuvant chemotherapy (NAC) had a second detection after NAC and before radical cystectomy. CTCs dynamic change after NAC was analyzed. Prognostic value of in vivo CTCs detection was investigated.

Results

Among 68 patients who received NAC, 45 patients (66%) had a CTC reduction after NAC. CTC reduction instead of baseline CTC positivity was a key prognostic factor for better progression free survival (PFS) among all MIBC patients receiving NAC in Kaplan–Meier analysis (P < 0.01) and in both crude (HR 6.14, 95%CI 1.63–23.21) and adjusted regression model (HR 6.76, 95% CI 1.59–28.88). The AUC was 0.85.

Conclusion

Our study demonstrated the prognostic value of in vivo detection of CTCs. The dynamic change of CTCs count may help evaluate the efficacy of NAC.

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This work was supported by the National Natural Science Foundation of China (82173076, 82103485), Shanghai Natural Science Foundation (20Y11904900), Young Talent Project in Healthcare of Shanghai Municipal Health Commission (2022YQ014), Shanghai Hospital Development Center (SHDC2022CRD034) and Innovative Research Team of High-level Local Universities in Shanghai.

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Author notes

  1. Di Jin and Lei Qian contributed equally to this work.

Authors and Affiliations

  1. Department of Urology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 200127, Shanghai, China

    Jin Di, Qian Lei, Zhang Ruiyun, Yang Guoliang, Zhang Lianhua, Xue Wei, Cao Ming, Dong Liang & Chen Haige

  2. Department of Pathology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 200127, Shanghai, China

    Xia Jun

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  1. Jin Di
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  2. Qian Lei
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Contributions

Di Jin, Liang Dong, and Haige Chen designed studies, analyzed, and wrote the manuscript. Di Jin, Lei Qian, Jun Xia, and Ming Cao conducted the experiments and data analysis. Guoliang Yang, Ruiyun Zhang, Lianhua Zhang and Wei Xue collected the patient samples, follow-up information, and performed the clinical data analysis. Lei Qian and Liang Dong prepared figures and table. All authors reviewed and approved the final manuscript.

Corresponding authors

Correspondence to Cao Ming, Dong Liang or Chen Haige.

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Ethical approval

The study complies with the Declaration of Helsinki and was conducted following the approval from Renji Hospital Ethics Committee. Written informed consent was obtained from all the patients.

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The authors have no competing interests to declare that are relevant to the content of this article.

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Jin, D., Qian, L., Xia, J. et al. In vivo detection demonstrates circulating tumor cell reduction instead of baseline number has prognostic value in bladder cancer patients receiving neoadjuvant chemotherapy. Cell Oncol. 46, 793–797 (2023). https://doi.org/10.1007/s13402-023-00785-x

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