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Cellular Oncology

, Volume 39, Issue 1, pp 35–45 | Cite as

HepG2 cells acquire stem cell-like characteristics after immune cell stimulation

  • Hang Wang
  • Miqing Yang
  • Ling Lin
  • Hongzhen Ren
  • Chaotong Lin
  • Suling Lin
  • Guoying Shen
  • Binfeng Ji
  • Chun MengEmail author
Original Paper

Abstract

Background

The presence of cancer stem cells (CSCs) is currently regarded as one of the main culprits of tumor formation and therapy failure. It is known that chronic inflammation is associated with CSCs, but it is not clear yet how inflammation affects the development of CSCs. In the present study we aimed to examine the relationship between cancer cell stimulation mediated by immune cells and the acquisition of a CSC-like phenotype.

Methods

Cancer cells derived from single hepatocarcinoma HepG2 cells were treated with mouse splenic B cells (MSBCs) and mouse peritoneal macrophage cells (MPMCs), respectively. The stem cell-like characteristics of the resulting HepG2 cells (MSBC-HepG2 and MPMC-HepG2) were evaluated using different assays, including biomarker assays, in vitro tumoroid and colony forming assays, in vivo tumor forming assays and signal transduction pathway activation assays.

Results

Various stemness characteristics of HepG2 cells, including self-renewal, proliferation, chemoresistance and tumorigenicity were evaluated. The expression levels of stemness-related genes and its encoded proteins in the MSBC-HepG2 and MPMC-HepG2 cells were assessed using RT-PCR and FACS analyses. We found that MSBC-HepG2 and MPMC-HepG2 cells possess hepatic CSC properties, including persistent self-renewal, extensive proliferation, drug resistance, high tumorigenic capacity and over-expression of CSC-related genes and proteins (i.e., EpCAM, ALDH, CD133 and CD44), compared to the parental cells. We also found that 1x103 MSBC-HepG2 and MPMC-HepG2 cells were able to form tumors in NOD/SCID mice and that the Notch and SHH signaling pathways were highly activated in MSBC-HepG2 cells.

Conclusions

We conclude that the immune system may have a double-edge effect on cancer development. On one hand, immune cells such as B lymphocytes and macrophages may recognize, attack and eliminate cancer cells, whereas on the other hand, they may promote a subset of cancer cells to acquire stem cell-like characteristics.

Keywords

Cancer stem cells HepG2 cells Splenic B cells Peritoneal macrophage cells 

Notes

Acknowledgments

This study was supported by the Fujian Minfaigaoji foundation (NO. 2014–514), the Fujian Education Office (NO. JA12033) and the Fuzhou University (NO.2013-XQ-27), Fujian province, P.R. China.

Compliance with ethical standards

Competing interests

The authors have declared that no competing interests exist.

Supplementary material

13402_2015_249_MOESM1_ESM.doc (29 kb)
Table S1 (DOC 29 kb)
13402_2015_249_MOESM2_ESM.docx (87 kb)
Figure S1 (DOCX 87 kb)
13402_2015_249_MOESM3_ESM.doc (34.1 mb)
Figure S2 (DOC 34926 kb)

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Copyright information

© International Society for Cellular Oncology 2015

Authors and Affiliations

  • Hang Wang
    • 1
  • Miqing Yang
    • 1
  • Ling Lin
    • 1
  • Hongzhen Ren
    • 1
  • Chaotong Lin
    • 1
  • Suling Lin
    • 1
  • Guoying Shen
    • 1
  • Binfeng Ji
    • 1
  • Chun Meng
    • 1
    • 2
    Email author
  1. 1.Institute of Pharmaceutical Biotechnology and Engineering, College of Biological Science and BiotechnologyFuzhou UniversityFuzhouChina
  2. 2.FuzhouPeople’s Republic of China

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