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Role of LOXs and COX-2 on FAK activation and cell migration induced by linoleic acid in MDA-MB-231 breast cancer cells

Cellular Oncology volume 36, pages 65–77 (2013)Cite this article

Abstract

Background

Epidemiological studies and animal models suggest a link between high levels of dietary fat intake and an increased risk of developing breast cancer. Particularly, free fatty acids (FFAs) are involved in several processes, including proliferation, migration and invasion, in breast cancer cells. Linoleic acid (LA) is a dietary n-6 polyunsaturated fatty acid that is known to induce proliferation and invasion in breast cancer cells. So far, however, the contribution of LA to focal adhesion kinase (FAK) activation and cell migration in breast cancer cells has not been studied.

Results

Here, we show that LA promotes FAK and Src activation, as well as cell migration, in MDA-MB-231 breast cancer cells. FAK activation and cell migration require Src, Gi/Go, COX-2 and LOXs activities, whereas both are independent of Δ6 desaturase activity. In addition, we show that cell migration requires FAK activity, whereas FAK activation requires Src activity, thus suggesting a reciprocal catalytic activation mechanism of FAK and Src.

Conclusions

In summary, our findings show that LA induces FAK activation and cell migration in MDA-MB-231 breast cancer cells.

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Acknowledgements

We are grateful to Teresa Robledo, Nora Ruiz and Catalina Flores for their technical assistance. This work was supported by a grant from CONACYT (83802) and UC-Mexus. N. S-M, S. V-C and O. G-H are supported by a CONACYT predoctoral training grant.

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The authors declare that they have no competing interests

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  1. Departamento de Biología Celular, Cinvestav-IPN, Av. IPN # 2508. San Pedro Zacatenco, Mexico, DF, 07360, Mexico

    Nathalia Serna-Marquez, Socrates Villegas-Comonfort, Octavio Galindo-Hernandez, Napoleon Navarro-Tito & Eduardo Perez Salazar

  2. Unidad de Investigación Especializada en Microbiologia, UAGRO, Mexico, Mexico

    Alejandro Millan

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  1. Nathalia Serna-Marquez
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Correspondence to Eduardo Perez Salazar.

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Serna-Marquez, N., Villegas-Comonfort, S., Galindo-Hernandez, O. et al. Role of LOXs and COX-2 on FAK activation and cell migration induced by linoleic acid in MDA-MB-231 breast cancer cells. Cell Oncol. 36, 65–77 (2013). https://doi.org/10.1007/s13402-012-0114-4

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Keywords

  • Breast cancer
  • Linoleic acid
  • FAK
  • Migration
  • COX-2
  • LOXs