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One-step enzymatic hydrolysis of sweet potato residue after gelatinization for bioethanol production by Saccharomyces cerevisiae

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Abstract

Sweet potato residue (SPR), a by-product of sweet potato starch extraction, is full of starch and cellulose and could be used as the starting material for bioethanol production. A novel one-step complex enzyme (including α-amylase, glucoamylase, cellulase, hemicellulase, and pectinase) hydrolysis approach was developed to liberate the fermentable carbohydrates present in SPR. The effects of pH, amount of enzymes, solid-to-liquid ratio, temperature, and enzyme reaction time on the reducing sugar yield were investigated. Experiment results showed that the optimum pH, solid-to-liquid ratio, amount of enzymes, enzymatic hydrolysis temperature, and time were 4.5, 1: 7, 0.32 g, 50 ℃, and 6 h, respectively. Under these optimum conditions, the experimental reducing sugar yield reached 65.06% ± 1.62%. Carbohydrate analysis of the enzymatic SPR showed that glucose accounted for the largest proportion of fermentable sugars at 58.91% ± 1.25%. In particular, 64.98% ± 0.11% of the cellulose was decomposed during the enzymatic hydrolysis. Finally, a concentration of 113.63 ± 1.35 g/L glucose was formed from the 17.2% (w/v) SPR substrate, and 46.9 ± 0.61 g/L ethanol was finally produced by an industrial diploid Saccharomyces cerevisiae strain at a yield of 27.27% ± 0.30% SPR. The proposed approach has great potential for industrial bioethanol production from SPR due to its high productivity, easy manipulation, and environmentally friendly characteristics.

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Funding

This work was supported by the Doctoral Scientific Research Start–up Foundation from Henan University of Technology (No. 2018BS072), the Key scientific research projects of universities in Henan Province (No. 22A180015), and The Open competition Research Projects of Xuchang University (No. 2022JBGS06).

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Authors and Affiliations

  1. College of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, Henan, China

    Chenchen Gou, Xiao Wang, Yuxin Yu, Jihong Huang, Xin Wang & Ming Hui

  2. School of Food and Pharmacy, Xuchang University, Xuchang, 461000, China

    Jihong Huang & Xin Wang

  3. Henan Provincial Engineering Laboratory of Preservation and Breeding of Industrial Microbial Strains, Zhengzhou, 450001, China

    Jihong Huang, Xin Wang & Ming Hui

  4. Henan Provincial Key Laboratory of Biological Processing and Nutritional Function of Wheat, College of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China

    Jihong Huang, Xin Wang & Ming Hui

  5. State Key Laboratory of Crop Stress Adaptation and Improvement, College of Agriculture, Henan University, Kaifeng, 475004, China

    Jihong Huang

Authors
  1. Chenchen Gou
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  2. Xiao Wang
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  3. Yuxin Yu
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  4. Jihong Huang
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  5. Xin Wang
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  6. Ming Hui
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Contributions

Chenchen Gou: investigation, writing—original draft preparation, and writing—reviewing and editing. Xiao Wang: formal analysis and writing—original draft preparation. Yuxin Yu: investigation and writing—original draft preparation. Jihong Huang: project administration. Xin Wang: writing—original draft preparation, writing—reviewing and editing, and funding acquisition. Ming Hui: supervision.

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Correspondence to Xin Wang or Ming Hui.

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Gou, C., Wang, X., Yu, Y. et al. One-step enzymatic hydrolysis of sweet potato residue after gelatinization for bioethanol production by Saccharomyces cerevisiae. Biomass Conv. Bioref. (2023). https://doi.org/10.1007/s13399-023-03755-3

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  • DOI: https://doi.org/10.1007/s13399-023-03755-3

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