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Green synthesis and characterization of CuO nanoparticles using Halymenia dilatata extract and its evaluation of antimicrobial, anticancer activity

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Abstract

The present investigation focused on the biosynthesis of copper oxide nanoparticles (CuONPs) using Halymenia dilatata seaweed aqueous extract. The biosynthesized CuONPs were successfully characterized using UV, FTIR, XRD, SEM, EDAX, HRTEM, and zeta potential analysis. The ultraviolet visible spectrum reflects maximum absorbance in the scale of 270 nm. The biosynthesized CuONPs had crystalline nature and were investigated using XRD. The FT-IR spectrum of CuONPs showed flavonoids and phenolic compounds could reduce the Cu2+ ions to CuONPs. SEM results indicated the nanoparticles were spherically shaped and 60 nm in size. The antimicrobial activity of H. dilatata mediated CuONPs was tested against six human pathogenic bacteria, and the highest growth inhibition was observed in B. subtillis (7.5 ± 0.4), S. aureus (6.8 ± 0.1), and K. pneumoniae (5.4 ± 0.8). The anticancer activity of HeLa cell line indicates a significant outcome with IC50 value (147.51 lg/ml) after 48 h treatment.

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Acknowledgements

The first author acknowledges Sri Sivasubramaniya Nadar College of Engineering, Chennai for providing Post-Doctoral Fellowship (SSN-PDF) (Ref. No. SSN CE PDF/2021). This research was funded by Princess Nourah bint Abdulrahman University Researchers Supporting Project Number (PNURSP2022R42), Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia. The authors also thank the Department of Chemical Engineering, Sri Sivasubramaniya Nadar College of Engineering, Chennai -603110, Tamil Nadu, India, for providing infrastructural facility.

Funding

This research was funded by Princess Nourah bint Abdulrahman University Researchers Supporting Project Number (PNURSP2022R42), Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.

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Authors and Affiliations

  1. Department of Botany, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India

    Saroja Ramasubbu Sivakumar

  2. Department of Chemical Engineering, Sri Sivasubramaniya Nadar College of Engineering, Chennai, 603 110, Tamil Nadu, India

    Kumar Manimaran

  3. Department of Materials Engineering, Ming Chi University of Technology, Taishan, 243303, Taiwan

    Mani Govindasamy

  4. International Ph.D. Program in Innovative Technology of Biomedical Engineering and Medical Devices, Ming Chi University of Technology, New Taipei City, 243303, Taiwan

    Mani Govindasamy

  5. Department of Research and Innovation, Saveetha School of Engineering, SIMATS, Chennai, 602105, India

    Mani Govindasamy

  6. Department of Chemistry, College of Science, Princess Nourah bint Abdulrahman University, P. O. Box 84428, Riyadh, 11671, Saudi Arabia

    Fatimah Mohammed Alzahrani & Norah Salem Alsaiari

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  1. Saroja Ramasubbu Sivakumar
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  2. Kumar Manimaran
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Contributions

Kumar Manimaran: Investigation, Conceptualization, Methodology, Writing-original draft. Saroja Ramasubbu Sivakumar: Formal analysis, Data curation. Mani Govindasamy: Formal analysis, Data curation. Fatimah Mohammed Alzahrani and Norah Salem Alsaiari: Formal analysis, Data curation.

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Correspondence to Kumar Manimaran.

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Sivakumar, S.R., Manimaran, K., Govindasamy, M. et al. Green synthesis and characterization of CuO nanoparticles using Halymenia dilatata extract and its evaluation of antimicrobial, anticancer activity. Biomass Conv. Bioref. (2023). https://doi.org/10.1007/s13399-022-03678-5

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