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Synergistic effect of glucose and glycerol accelerates microbial lipid production from low-cost substrates by Cutaneotrichosporon oleaginosum

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Abstract

Efficient conversion of various low-cost substrates into lipid is pivotal for better techno-economics of the microbial lipid technology. In this study, lipid production by Cutaneotrichosporon oleaginosum from glucose, glycerol, and their mixture at various mass ratios was investigated. Co-existence of glucose and glycerol rendered higher lipid productivity and yield, supporting synergetic effects of the two agents. Interestingly, the theoretical lipid yield was not improved through combination utilization of glucose and glycerol based on the prediction using a small-scale metabolic model. The synergy was probably owing to the improvement of substrate transportation efficiency through simultaneous assimilation of glucose and glycerol. When waste paper enzymatic hydrolysates and crude glycerol were co-utilized, lipid content, yield, and productivity attained 62.3%, 18.9 g/100 g, and 4.1 g/L/d, separately. The lipid samples showed fatty acid compositions comparable to that of rapeseed oil. The prediction of biodiesel properties demonstrated the feasibility for high-quality fuel production. These results showed that the co-fermentation strategy could serve as an efficient way to advance microbial lipid production, which has bright prospects expanding to low-cost substrates including cellulosic biomass and crude glycerol.

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Data availability

The data used to support the findings of this study are available from the corresponding author upon request.

Abbreviations

ATCC:

The American Type Culture Collection

YPD:

Yeast peptone dextrose

FPU:

Filter paper unit

CBU:

Cellobiase unit

C/N:

Carbon/nitrogen ratio

Glc:

Glucose

Glyc:

Glycerol

KV:

Kinematic viscosity

D:

Density

HHV:

The higher heating value

SV:

Saponification value

IV:

Iodine value

CN:

Cetane number

CFPP:

Cold filter plugging point

OS:

Oxidative stability

DU:

Degree of unsaturation

LCSF:

Long-chain saturation factor

ANOVA:

An analysis of variance

PPP:

Pentose phosphate pathway

TCA:

Tricarboxylic acid cycle

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Funding

This research was funded by the National Natural Science Foundation of China (51608400) and the Open Funding Project of the State Key Laboratory of Biocatalysis and Enzyme Engineering (SKLBEE2018015).

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, 947 Heping Road, Wuhan, 430081, People’s Republic of China

    Zhiwei Gong, Man Zhao, Wei Zhou, Mou Tang & Wenting Zhou

  2. State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062, China

    Qiaoning He

  3. HuBei Province Key Laboratory of Coal Conversion and New Carbon Materials, Wuhan University of Science and Technology, 430081, Wuhan, People’s Republic of China

    Zhiwei Gong & Wenting Zhou

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  1. Zhiwei Gong
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Correspondence to Zhiwei Gong or Wenting Zhou.

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Gong, Z., Zhao, M., He, Q. et al. Synergistic effect of glucose and glycerol accelerates microbial lipid production from low-cost substrates by Cutaneotrichosporon oleaginosum. Biomass Conv. Bioref. 14, 859–867 (2024). https://doi.org/10.1007/s13399-022-02369-5

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