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Ultrasound-assisted extraction of phytochemicals from Empetrum hermafroditum Hager. using acid-based deep eutectic solvent: kinetics and optimization

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Abstract

The present study has applied acid-based deep eutectic solvent containing choline chloride with malonic, malic, tartaric, and citric acids for ultrasound-assisted extraction of bioactive compounds from Empetrum hermafroditum Hager. Kinetics of the extraction process has been estimated in the framework of the second-order and film theory models obtaining time dependence of total phenolic compound yield and total flavonoid yield using Folin-Ciocalteu and AlCl3-complexation methods. Total antioxidant activity of extracts has been estimated using the phosphomolybdate method and DPPH radical assay. HPLC has determined that quercetin and kaempferol were present in the extracts. Temperature, time, and water addition to deep eutectic solvents (DESs) have been optimized for the extraction process using the Box-Behnken design. The optimal conditions for the extraction of polyphenolic compounds are 3 h at 45 °C with the addition of 50% water. Polyphenol yield achieves 320.8 mg mL−1. The optimal conditions for flavonoid extraction are 5 h at 60 °C with the addition of 30% water. Total flavonoid yield achieves 58.8 mg mL−1. Obtained data demonstrate DESs as promising extractants for bioactive component recovery from Empetrum hermafroditum Hager.

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Acknowledgements

The experimental work was facilitated by the equipment of Magnetic Resonance Research Centre at St. Petersburg State University.

Funding

This work was carried out with the financial support of the Grant of the President of the Russian Federation (MK-566.2020.11), within the framework of Research Contracts No. FMEZ-2022–0020 and 0186–2021-0019 provided by the Ministry of Science and Higher Education of the Russian Federation and within the State Task of the research topic No. AAAA18-118021490070–5.

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Authors and Affiliations

  1. I.V. Tananaev Institute of Chemistry and Technology of Rare Elements and Mineral Raw Materials - Subdivision of the Federal Research Centre, Kola Science Centre of the Russian Academy of Sciences, Apatity, Russia

    Nikita Tsvetov

  2. Federal Research Centre, Laboratory of Medical and Biological Technologies, Kola Science Centre of the Russian Academy of Sciences, Apatity, Russia

    Lidia Sereda & Anna Korovkina

  3. Institute of North Industrial Ecology Problems – Subdivision of the Federal Research Centre, Kola Science Centre of the Russian Academy of Sciences, Apatity, Russia

    Natalia Artemkina

  4. Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Moscow, Russia

    Irina Kozerozhets

  5. Department of Chemical Thermodynamics and Kinetics, Saint Petersburg State University, St. Petersburg, Russia

    Artemiy Samarov

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  1. Nikita Tsvetov
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Correspondence to Nikita Tsvetov.

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Tsvetov, N., Sereda, L., Korovkina, A. et al. Ultrasound-assisted extraction of phytochemicals from Empetrum hermafroditum Hager. using acid-based deep eutectic solvent: kinetics and optimization. Biomass Conv. Bioref. 12 (Suppl 1), 145–156 (2022). https://doi.org/10.1007/s13399-022-02299-2

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