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Photobiostimulation of green microalga Chlorella sorokiniana using He–Ne red laser radiation for increasing biodiesel production

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Abstract

Microalga would be the paramount resource of biodiesel able of satisfying the world requirements for transportation fuels, which could fully replace the petrodiesel. Therefore, the research studies focus on developing novel biodiesel production methods. The present study investigates the effect of monochromatic light such as red light-emitting diodes (LEDs) and He–Ne red laser radiation on the accumulated lipid and the growth of the green microalgae Chlorella sorokiniana. The irradiation of microalgal cells with He–Ne red laser source which has a wavelength of 632.8 nm was hypothesized to enhance the accumulation of lipid inside the algal cells, which ultimately increases the biodiesel production. The photobiostimulating effects of laser irradiation on biodiesel was investigated by irradiating the microalga for a duration of 2 h with 632.8 nm He–Ne red laser source compared with 2 h irradiation with red LEDs and 2 h irradiation with white light (the control). The results showed that the oil content inside the algal cells irradiated with He–Ne red laser was 3.1 times the algal cells irradiated with white light (the control). Similarly, the biodiesel yielded from the algal cells irradiated with He–Ne red laser was 3.1 times the biodiesel yielded from the algal cells irradiated with white light (the control). However, the oil content and the biodiesel yield from algal cells irradiated with red LEDs were only 0.82 times those of the control. Therefore, it was concluded that the irradiation of microalga with red laser increases the biodiesel yield.

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Abbreviations

LEDs:

Light-emitting diodes

DW:

Dry weight

FW:

Fresh weight

PBR:

Photobioreactor

LMS:

Longitudinal mode spacing

CURP:

Cairo University Research Park

AOAC:

Association of Official Agricultural Chemists

ANOVA:

Analysis of variance

PCA:

Principle component analysis

Mean diff:

Mean difference

SEM:

Standard error of mean

Prob:

Probability

Sig:

Significance

LCL:

Lower control limit

UCL:

Upper control limit

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Funding

This study is funded by the Science and Technology Development Fund (STDF) of Egypt through the research project number 26272.

Author information

Authors and Affiliations

  1. Department of Agricultural Engineering, Faculty of Agriculture, Cairo University, Giza, Egypt

    M. Faried & M. Samer

  2. Department of Microbiology, Faculty of Agriculture, Cairo University, Giza, Egypt

    M. A. Moselhy, A. S. Ali & R. H. Ahmed

  3. Department of Biochemistry, Faculty of Agriculture, Cairo University, Giza, Egypt

    R. S. Yousef

  4. Department of Food Toxicology and Contaminants, Marine Toxins Laboratory, National Research Centre, Giza, Egypt

    D. A. Marrez

  5. Department of Laser Applications in Metrology, Photochemistry and Agriculture, National Institute of Laser Enhanced Sciences, Cairo University, Giza, Egypt

    A. El-Hussein & E. M. Abdelsalam

  6. Faculty of Science, Galala University, Suez, Egypt

    A. El-Hussein

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  1. M. Faried
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  2. M. Samer
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  3. M. A. Moselhy
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  4. R. S. Yousef
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  5. A. S. Ali
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  8. A. El-Hussein
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  9. E. M. Abdelsalam
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Correspondence to M. Samer or E. M. Abdelsalam.

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Highlights

• Effects of He–Ne red laser on accumulated lipid and algae growth were studied

• Algae irradiation by red laser with a wavelength of 632.8 nm raises biodiesel yield

• Algae irradiation by He–Ne red laser raises oil content 3.1 times the control

• Algae irradiation by He–Ne red laser raises biodiesel yield 3.1 times the control

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Faried, M., Samer, M., Moselhy, M.A. et al. Photobiostimulation of green microalga Chlorella sorokiniana using He–Ne red laser radiation for increasing biodiesel production. Biomass Conv. Bioref. 14, 117–131 (2024). https://doi.org/10.1007/s13399-021-02220-3

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