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Conversion of cassava peels into bioethanol using the OSTEP approach

Biomass Conversion and Biorefinery (2022)Cite this article

Abstract

Bioethanol from lignocellulosic biomass poses as a sustainable alternative to gasoline. However, the high cost of pre-treatment and hydrolysis of lignocellulosic biomass tends to limit their utilization as feedstock for ethanol biorefinery. This study therefore sought to convert cassava (CRI-Abrabopa) peels into ethanol using the on-site tailored enzyme production approach. Fungi were isolated from CRI-Abrabopa peels by the spread and direct plate techniques. Isolates obtained were subjected to morphological identification and molecular characterization by sequencing ITS spacer genes (rRNA). On-site tailored enzymes were produced from the most potent lignocellulolytic fungus. On-site and off-site (CTEC 2) enzymes were used to hydrolyse peels before fermenting to ethanol using Saccharomyces cerevisiae. CRI-Abrabopa peels were found to be a highly efficient feedstock for bioethanol production due to their rich-sugar content (78.3% dry biomass). Molecular characterization of the most potent lignocellulolytic isolate revealed it as Trametes polyzona BKW-001. Its profile revealed the presence of cellulases, xylanases, and amylases, with the amylase activity being the most pronounced at 56.2 U/mL. The on-site tailored enzyme production approach used in this study proved to be an efficient alternative to the commercial off-site enzyme preparation with a comparative sugar recovery efficiency of up to 90%. An ethanol yield of 28.8 g/100 g reducing sugars (on-site enzyme derived) was obtained from the cassava peels. Findings from this study have demonstrated the suitability and possible adoption of on-site tailored enzyme application in conventional ethanol biorefinery.

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All data obtained or analysed during this study are included in this published article.

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Funding

This study was funded by DANIDA Fellowship Centre (Grant Number 14-01DTU).

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Authors and Affiliations

  1. Department of Microbiology, University for Development Studies, Nyankpala, Ghana

    Nana Aboagye Acheampong

  2. Department of Theoretical and Applied Biology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana

    William Gariba Akanwariwiak

  3. Department of Marine Engineering, Regional Maritime University, Accra, Ghana

    Moses Mensah & Felix Offei

  4. Department of Environmental Science, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana

    Bernard Fei-Baffoe

  5. Department of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana

    Joseph Asankomah Bentil

  6. Department of Environment, Water and Waste Engineering, University for Development Studies, Nyankpala, Ghana

    Abubakari Zarouk Imoro

  7. Department of Chemistry, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana

    Lawrence Sheringham Borquaye

  8. Central Laboratory, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana

    Lawrence Sheringham Borquaye

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  1. Nana Aboagye Acheampong
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  2. William Gariba Akanwariwiak
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  3. Moses Mensah
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Contributions

MYM, LSB, WGA, BF-B, and NAA conceived the study. MYM secured financial support for the study. NAA isolated the strains and conducted all the activity assays. JAB assisted with study design and molecular characterization of the strain. NAA wrote the manuscript. LSB, AZI, and FO revised the manuscript. WGA and BF-B supervised the study. NAA, AZI, and FO conducted analytical and statistical analyses.

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Correspondence to Lawrence Sheringham Borquaye.

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Highlights

• Peels of CRI-Abrabopa proved to be a reliable source of hydrolytic enzymes and suitable feedstock for ethanol production.

• T. polyzona (BKW-001) exhibited profound ligninolytic, cellulolytic, and hemicellulolytic abilities.

• On-site tailored enzymes from CRI-Abrabopa cassava peels were a highly efficient alternative to commercial enzymes for biomass hydrolysis.

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Acheampong, N.A., Akanwariwiak, W.G., Mensah, M. et al. Conversion of cassava peels into bioethanol using the OSTEP approach. Biomass Conv. Bioref. (2022). https://doi.org/10.1007/s13399-021-02149-7

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  • DOI: https://doi.org/10.1007/s13399-021-02149-7

Keywords

  • Manihot esculenta
  • Trametes polyzona
  • Lignocellulolytic fungi
  • On-site enzymes
  • Biofuel production