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Biochemical and molecular identification of lipolytic bacteria isolated from beverage industrial wastewater and optimization of lipase-secreting bacteria

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Abstract

This study aims to isolate and identify lipase-producing bacteria from wastewater of beverage industries, Khurda industrial estate, Odisha. Based on biochemical screening, Pseudomonas sp. (strain no. 26.1), Staphylococcus sp. (strain no. 29.1 and strain no. 29.3), and Bacillus sp. (strain no. 34.1) were identified respectively as lipase-producing strains. Bacillus sp. (strain no. 34.1) was identified as the most efficient lipase enzyme producer (8 mm) based on the lipase chromogenic plate assay (LCA). Later, the most efficient lipase enzyme producer (strain no. 34.1) was identified as Bacillus cereus (GenBank Accession number: MZ410629) based on 16S rRNA sequencing. In the current study, the activity of the lipase enzyme was observed at 50° C, pH 7.0, and in the presence of 1 mM Ca2+. Bacillus cereus (strain no. 34.1) started lipase secretion at pH 6 ± 0.5 at 37 °C for 12–16 h and reached maximum secretion at 46–48 h in the pilot-scale fermenter. The lipase enzyme molecular weight of Bacillus cereus and fermenter sample (FS) was about 44 kDa and 47 kDa respectively by SDS-PAGE. The TLC chromatographic picture of Bacillus cereus showed only triglycerides but fermenter strain (FS) showed intermediate hydrolysis products such as monoglycerides, diglycerides, free fatty acids, and triglycerides compared to control (oleic acid). In conclusion, it can be inferred from the current study that wastewater from beverage industries is used as a good source of lipase-producing bacteria and could be used for potential industrial significance. Hence, Bacillus cereus could be used as a potential candidate for lipase production as well as for bioremediation agents for the treatment of oil-contaminated wastewater.

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Acknowledgements

The authors acknowledge the Department of Biotechnology, Academy of Management & Information Technology, and North Orissa University for providing lab and instrument facilities to carry out the present work.

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Authors and Affiliations

  1. Department of Zoology, Mizoram University, Aizawl, 796004, Mizoram, India

    Abinash Giri, Pratima Khandayataray & Meesala Krishna Murthy

  2. Department of Biotechnology, Academy of Management & Information Technology, 67, Vidya Vihar, IID Center, Near Barunei Hills, Khurda, Orissa, 752055, India

    Dibyaranjan Samal

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  1. Abinash Giri
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Giri, A., Khandayataray, P., Murthy, M.K. et al. Biochemical and molecular identification of lipolytic bacteria isolated from beverage industrial wastewater and optimization of lipase-secreting bacteria. Biomass Conv. Bioref. 13, 10723–10734 (2023). https://doi.org/10.1007/s13399-021-01890-3

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