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Development of consolidated bioprocess for biofuel-ethanol production from ultrasound-assisted deep eutectic solvent pretreated Parthenium hysterophorus biomass

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Abstract

A novel consolidated bioprocess was designed for processing of Parthenium hysterophorus biomass based on ultrasound-assisted deep eutectic solvent (DES) pretreatment followed by in situ enzymatic saccharification and fermentation of sugars to ethanol-biofuel. Among various DESs examined, choline chloride/sorbitol (CCS, molar ratio 1:5) pretreated P. hysterophorus biomass yielded maximum sugar (148.54 mg/g biomass) upon enzymatic saccharification. Furthermore, combined ultrasonic irradiation and CCS pretreatment resulted in substantially enhanced sugar yield as compared with either of the standalone pretreatment. A central composite design was employed for the optimization of combined ultrasound and choline chloride/sorbitol pretreatment that further aided in achieving increased reducing sugar yield (25.72%). The pretreated biomass was examined by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and proton nuclear magnetic resonance (1H-NMR) analysis to elucidate the functional mechanisms of combinative pretreatment. Understanding of pretreatment mechanisms may help custom designing of novel strategies for the efficient refining of biomass.

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Acknowledgements

Authors thankfully acknowledge the Director, School of Biotechnology, University of Jammu, Jammu, for providing necessary laboratory facilities. Professor Raj K. Rampal, Head, Department of Environmental Sciences, and Ms Nishu, Research Scholar, are thanked for kindly helping in lignin analysis in the biomass. SAIF, STIC, Cochin University of Science and Technology, Kerala, India, and SAIF, IIT Madras, Chennai, India, are acknowledged for physicochemical analysis.

Availability of data and material

All datasets used and analyzed in the current study are available from the corresponding author.

Funding

Dr. Bijender Kumar Bajaj gratefully acknowledges the Institute of Advanced Study, Durham University, UK, for providing COFUND International Senior Research Fellowship for a research stay at the Department of Biosciences, Durham University, Durham, UK, and the Commonwealth Scholarship Commission, UK, for providing Commonwealth Fellowship for a research stay at the Institute of Biological, Environmental and Rural Sciences (IBERS), Aberystwyth University, Aberystwyth, UK, and Indo-US Science and Technology Forum (IUSSTF) for a research stay at the Ohio State University, USA. Financial support in the form of Research Projects to Dr. Bijender Kumar Bajaj (B.K.B.) from funding agencies such as the Department of Science and Technology (DST), University Grants Commission (UGC) and Council of Scientific and Industrial Research (CSIR) is gratefully acknowledged. Ms. Parushi Nargotra acknowledges Rashtriya Uchchattar Shiksha Abhiyan (RUSA) for providing a Ph.D. research fellowship. Mr. Vishal Sharma thankfully acknowledges the Department of Science and Technology (Govt. of India) for providing Inspire fellowship for doctoral research. Ms. Surbhi Sharma is thankful to the University of Jammu for providing the University Research Scholarship.

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Authors and Affiliations

  1. School of Biotechnology, University of Jammu, Jammu, India

    Parushi Nargotra, Vishal Sharma, Surbhi Sharma, Nisha Kapoor & Bijender Kumar Bajaj

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  1. Parushi Nargotra
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  2. Vishal Sharma
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  3. Surbhi Sharma
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  5. Bijender Kumar Bajaj
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Contributions

Dr. Bijender Kumar Bajaj (B.K.B.) conceptualized and designed the research problem. Material preparation, data collection, analysis, investigation and interpretation were performed by Ms. Parushi Nargotra (P.N.) and Mr. Vishal Sharma (V.S.). P.N. wrote the first draft of the manuscript. V.S. and Ms. Surbhi Sharma (S.S.) participated in the editing and preparation of the final manuscript. B.K.B. and Ms. Nisha Kapoor (N.K.) critically reviewed the manuscript for grammatical and scientific errors. B.K.B. submitted the MS. All authors read and approved the final manuscript.

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Correspondence to Bijender Kumar Bajaj.

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Highlights

• First report on bioprocessing of P. hysterophorus using deep eutectic solvents, DESs.

• Ultrasound-assisted DES pretreatment enhances the sugar yield and hence the process efficacy.

• A one-pot consolidated process was developed for combined DES and ultrasound pretreatment.

• Molecular mechanism of pretreatment was studied by SEM, FT-IR, XRD and 1H-NMR

• Consolidated one-pot bioprocess for the conversion of biomass to biofuel-ethanol.

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Nargotra, P., Sharma, V., Sharma, S. et al. Development of consolidated bioprocess for biofuel-ethanol production from ultrasound-assisted deep eutectic solvent pretreated Parthenium hysterophorus biomass. Biomass Conv. Bioref. 12, 5767–5782 (2022). https://doi.org/10.1007/s13399-020-01017-0

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