A quick and effective method for evaluating substrate-enzyme systems in the enzymatic hydrolysis of lignocellulosic biomass
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This work describes a microreaction technique to carry out hydrolysis of certain substrate by certain enzyme under several conditions, and reports its usefulness to determine effective process conditions and to compare performances between different systems substrate-enzyme, in a quick and economic way. The microreaction system consists of microtubes, as microreactors, that are placed into a thermomixer, allowing a set of 24 experiments at a certain constant temperature but with different pH and substrate-enzyme ratios. The outcomes of the numerous experiments are processed via response surface methodology and means analysis. In order to illustrate the technique effectiveness, it is discussed the comparison of two commercial enzyme complexes, and two delignification procedures of wheat straw.
KeywordsEnzymatic hydrolysis Enzymatic process design High-throughput screening Lignocellulosic biomass Second-generation bioethanol
Financial support from Secretary of Energy (SENER), México (Project SENER 2009-150001) is acknowledged.
- 1.Parajuli R, Dalgaard T, Jørgensen U, Adamsen APS, Knudsen MT, Birkved M, Gylling M, Schjørring JK (2015) Biorefining in the prevailing energy and materials crisis: a review of sustainable pathways for biorefinery value chains and sustainability assessment methodologies. Renew Sust Energ Rev 43:244–263. https://doi.org/10.1016/j.rser.2014.11.041 CrossRefGoogle Scholar
- 11.Marcos M, García-Cubero MT, González-Benito G, Coca M, Bolado S, Lucas S (2012) Optimization of the enzymatic hydrolysis conditions of steam-exploded wheat straw for maximum glucose and xylose recovery. J Chem Technol Biotechnol 88(2):237–246. https://doi.org/10.1002/jctb.3820 CrossRefGoogle Scholar
- 13.Adney B, Baker J (1996) Measurement of cellulase activities, Laboratory Analytical Procedure, National Renewable Energy Laboratory, Technical Report NREL/TP 510-42628Google Scholar
- 14.Martins LF, Kolling D, Camassola M, Dillon AJP, Ramos LP (2008) Comparison of Penicillium echinulatum and Trichoderma reesei cellulases in relation to their activity against various cellulosic substrates. Bioresour Technol 99(5):1417–1424. https://doi.org/10.1016/j.biortech.2007.01.060 CrossRefGoogle Scholar
- 23.Jäger G, Wulfhorst H, Zeithammel EU, Elinidou E, Spiess AC, Büchs J (2011) Screening of cellulases for biofuel production: online monitoring of the enzymatic hydrolysis of insoluble cellulose using high-throughput scattered light detection. Biotechnol J 6(1):74–85. https://doi.org/10.1002/biot.201000387 CrossRefGoogle Scholar
- 26.AOAC International (2005) Official methods of analysis, 18th edition, Method 973.18 Fiber (Acid Detergent) and Lignin in Animal Feed, Maryland USAGoogle Scholar
- 30.Dowe NS, Lussier JM, Gleason RM (2008) Novel method for the rapid simultaneous measurement of xylose and glucose for bioethanol production. www.ysi.com. Accessed 23 Aug 2017
- 32.Montgomery DC (2013) Design and analysis of experiments. New JerseyGoogle Scholar