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Green Fabrication of Copper Oxide Nanoparticles: A Comparative Antibacterial Study Against Gram-Positive and Gram-Negative Bacteria

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Abstract

The Greener synthesis of nanoparticles (NPs) is gaining importance due to its ease and economy. One-pot production of NPs utilizing vegetable peels is purely green. The current study reports the use of cauliflower (Brassica oleracea), potato (Solanum tuberosum), and pea (Pisum sativum) peels extract for the synthesis of copper oxide (CuO) NPs. An ecofriendly approach is established by performing synthesis in an aqueous medium, along with minimum use of chemicals, and low fabrication temperature. FTIR peaks for self-assembled Cu–O bond are found at 526 cm−1, 590 cm−1, and 582 cm−1 for calcined NPs made from cauliflower, potato, and pea extract accordingly. By using CuCl2·2H2O, the particle size of NPs is 32.5 nm, 40.7 nm, and 47.2 nm from cauliflower, potato, and pea peels, respectively. Moreover, differences in NPs shape like roughly spherical and cubical indicate the impact of phytochemicals during construction. Furthermore, this study is an exploration of the antibacterial efficacy of NPs. The inhibitory action of CuO NPs at 45 µg/mL was figured out as Pseudomonas aeruginosa > Escherichia coli > Bacillus subtilus > Staphylococcus aureus. A more significant biocidal effect of NPs was found on the growth of Gram-negative than Gram-positive bacteria. NPs are found superior for inhibition and inactivation of cell growth than NPs parent material against control. The current study presents biowaste management and its conversion to a useful product.

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Authors and Affiliations

  1. Department of Chemistry, Fatima Jinnah Women University, Rawalpindi, Pakistan

    Aliya Fazal

  2. Department of Biochemistry and Molecular Biology, University of Gujrat, Gujrat, Pakistan

    Sajida Ara & Kalsoom Sughra

  3. Department of Chemistry, National University of Sciences and Technology, Islamabad, Pakistan

    Muhammad Tayab Ishaq

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  1. Aliya Fazal
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  2. Sajida Ara
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Correspondence to Aliya Fazal.

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Fazal, A., Ara, S., Ishaq, M.T. et al. Green Fabrication of Copper Oxide Nanoparticles: A Comparative Antibacterial Study Against Gram-Positive and Gram-Negative Bacteria. Arab J Sci Eng 47, 523–533 (2022). https://doi.org/10.1007/s13369-021-05767-5

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