MFC—An Approach in Enhancing Electricity Generation Using Electroactive Biofilm of Dissimilatory Iron-Reducing (DIR) Bacteria
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A potential dissimilatory iron-reducing bacteria Klebsiella pneumoniae was employed in dual chamber microbial fuel cell for the formation of biofilm on the anode surface. Biofilm development on the electrode was examined as extracellular polymeric substances and phospholipids quantitatively. Significant increase in open circuit voltage and the current was observed from first cycle (0.950 V, 1.250 mA) to the last cycle (1.2 V, 1.683 mA) of microbial fuel cell operation. Increasing columbic efficiency from 8 to 62% showed the amount of electrons available from the oxidation of organic matter into electricity. Chemical oxygen demand removal efficiency increment from 44 to 85% establishes effective utilization of organic matter by K. pneumoniae. The scanning electron microscopic observations proved the ability to form a biofilm on an electrode surface. Results of the present study suggested that increasing power output is directly proportional to biofilm formed on the electrode surface. Biofilm development enhances the current production as a result of effective electrocatalysis by K. pneumoniae.
KeywordsBiofilm Microbial fuel cell COD Columbic efficiency K. pneumoniae
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- 1.Energy Information Administration, U.S. Department of Energy, Washington, Monthly Energy Review (2015). http://www.eia.gov/forecasts/aeo. Accessed 22 Apr 2017
- 7.Yuvraj, C.; Aranganathan, V.: Isolation and identification of prospective dissimilatory iron reducing bacteria for electricity generation in microbial fuel cell. Int. J Adv. Lif. Sci. 8(3), 300–306 (2015)Google Scholar
- 8.Yuvraj, C.; Aranganathan, V.: Enhancement of voltage generation using isolated dissimilatory iron-reducing (DIR) bacteria Klebsiella pneumoniae in microbial fuel cell. Arab. J. Sci. Eng. (2016). doi: 10.1007/s13369-016-2108-4
- 11.Lowry, O.H.; Rosebrough, N.J.; Farr, A.L.; Randall, R.J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193(1), 265–275 (1951)Google Scholar
- 12.Aelterman, P.; Freguia, S.; Keller, J.; Verstraete, W.; Rabaey, K.: The anode potential regulates bacterial activity in microbial fuel cells. Appl. Environ. Microbiol. 78(3), 409–418 (2008)Google Scholar
- 16.Findlay, R.H.; King, G.M.; Watling, L.: Efficacy of phospholipid analysis in determining microbial biomass in sediments. Appl. Environ. Microbiol. 55(11), 2888–2893 (1989)Google Scholar
- 18.Wicker-Böckelmann, U.; Wingender, J.; Winkler, U.K.: Alginate lyase releases cell-bound lipase from mucoid strains of Pseudomonas aeruginosa. Zbl. Bakt Int. J. Med. M. 266(3), 379–389 (1987)Google Scholar
- 21.Flemming, H.C.; Wingender, J.; Mayer, C.; Korstgens, V.; Borchard, W.: (2000). Cohesiveness in biofilm matrix polymers. In Symposia-Society for General Microbiology (pp. 87–106). Cambridge University Press, Cambridge (1999).Google Scholar
- 22.Baranitharan, E.; Khan, M.R.; Prasad, D.M.R.: Treatment of palm oil mill effluent in microbial fuel cell using polyacrylonitrile carbon felt as electrode. J Med. Biol. Eng. 2(4), 252–256 (2013)Google Scholar
- 23.Khater, D.; El-khatib, K.M.; Hazaa, M.; Hassan, R.Y.: Electricity generation using Glucose as substrate in microbial fuel cell. J. Bas. Environ. Sci. 2, 84–98 (2015)Google Scholar