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Ultrasound-Assisted Rapid Extraction of Bacterial Intracellular Medium-Chain-Length Poly(3-Hydroxyalkanoates) (mcl-PHAs) in Medium Mixture of Solvent/Marginal Non-solvent

  • Research Article - Biological Sciences
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Abstract

Intracellular medium-chain-length poly(3- hydroxyalkanoates) (PHAs) produced by Pseudomonas putida Bet001 were extracted in an ultrasound-assisted process. A mixture of acetone (solvent) and heptane (marginal non-solvent) was used as the extraction medium. The effects of volumetric energy dissipation, extraction medium ratio and irradiation time on the extraction process were investigated. Sonication frequency of 37 kHz and heptane as marginal non-solvent facilitated the process. Following optimization, high PHA extraction rate of \({74 \times 10^{-3}{\rm g}}\) PHA \({{\rm g}^{-1}}\) dried biomass \({{\rm min}^{-1}}\) was observed at ultrasonic energy output of \({1151 \pm 3 {\rm J ml}^{-1}}\) with 50:50 solvent/marginal non-solvent ratio for irradiation time of 5 min. PHA showed good stability under the rapid extraction process.

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Authors and Affiliations

  1. Institute of Biological Sciences, Faculty of Sciences, University of Malaya, 50603, Kuala Lumpur, Malaysia

    K. A. Ishak, M. S. M. Annuar & A. M. Gumel

  2. Department of Chemistry, Faculty of Sciences, University of Malaya, 50603, Kuala Lumpur, Malaysia

    T. Heidelberg

Authors
  1. K. A. Ishak
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  2. M. S. M. Annuar
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  3. T. Heidelberg
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  4. A. M. Gumel
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Correspondence to M. S. M. Annuar.

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Ishak, K.A., Annuar, M.S.M., Heidelberg, T. et al. Ultrasound-Assisted Rapid Extraction of Bacterial Intracellular Medium-Chain-Length Poly(3-Hydroxyalkanoates) (mcl-PHAs) in Medium Mixture of Solvent/Marginal Non-solvent. Arab J Sci Eng 41, 33–44 (2016). https://doi.org/10.1007/s13369-015-1833-4

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  • DOI: https://doi.org/10.1007/s13369-015-1833-4

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