Abstract
Rabies is a neurological disease with 100% lethality. Some of the rare human patients who survived after multiple drug treatment had severe sequelae. The present study showed that after 48 h of RABV inoculation, mice injected intracerebrally with anti-RABV F (ab’)2 plus Bioporter® showed 70% survival compared to the control group, suggesting that transfection of anti-RABV antibodies to the brain may prevent or delay the spread of RABV at an early stage of infection. This result may provide important protocol results in intracellular antibody delivery to prevent the fatal outcome of the disease.
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Funding
The authors are thankful for the financial support provided by CNPq (Brazilian National Board for Scientific and Technological Development) grant number 307291/2017-0 and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil) Finance Code 001, which had no role in the study design, collection, analysis and interpretation of data, writing of the report, and in the decision to submit the article for publication.
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This experiment was approved by the Ethics Committee on Animal Use (CEUA) of the School of Veterinary Medicine and Animal Science - University of São Paulo (FMVZ-USP), under protocol no. 9658071016. All mice were used for the experiments; prior to any procedure, mice were anesthetized with isofluoran.
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Agostinho, W.C., Brandão, P.E. Intracerebral transfection of anti-rabies virus antibodies is an effective therapy for rabies. J. Neurovirol. 26, 764–768 (2020). https://doi.org/10.1007/s13365-020-00883-8
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DOI: https://doi.org/10.1007/s13365-020-00883-8