Abstract
Encephalitis is an infrequent manifestation in the various spectrums caused by severe fever with thrombocytopenia syndrome virus (SFTSV) infection. There are few data about the possible pathogenic mechanisms of SFTSV-associated encephalitis. Here, two SFTSV-infected patients with onset of encephalitis were enrolled. The whole genome of two SFTSV strains isolated from cerebrospinal fluid (CSF) was deeply sequenced by next-generation sequencing (NGS) and phylogenetic analysis was conducted. The specific mutations of M fragment were P98L and T665S respectively. The three-dimensional structure of glycoprotein Gn which was encoded by M fragment, an important virulence factor of SFTSV, was constructed by SWISS-MODEL. There was no significant variation in glycoprotein Gn of the two isolates comparing to other strains without encephalitis. Phylogenetic trees based on the complete sequences of M segment showed the two strains were highly identical to other local strains without encephalitis. Our study demonstrates that the virulence factors of SFTSV with encephalitis are not different from those without encephalitis. SFTSV itself is a neurotropic virus.
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References
Antic D, Wright KE, Kang CY (1992) Maturation of Hantaan virus glycoproteins G1 and G2. Virology 189:324–328
Cain MD, Salimi H, Gong Y, Yang L, Hamilton SL, Heffernan JR et al (2017) Virus entry and replication in the brain precedes blood-brain barrier disruption during intranasal alphavirus infection[J]. J neuroimmunol 308(15):118–130. https://doi.org/10.1016/j.jneuroim.2017.04.008
Choi SJ, Park SW, Bae IG, Kim SH, Ryu SY, Kim HA et al (2016) Severe fever with thrombocytopenia syndrome in South Korea, 2013-2015. PloS Negl Trop Dis 10(12):e0005264. https://doi.org/10.1371/journal.pntd.0005264
Cui N, Liu R, Lu QB, Wang LY, Qin SL, Yang ZD, Zhuang L, Liu K, Li H, Zhang XA, Hu JG, Wang JY, Liu W, Cao WC (2015) Severe fever with thrombocytopenia syndrome bunyavirus-related human encephalitis. J Infect 70:52–59. https://doi.org/10.1016/j.jinf.2014.08.001
Deng B, Zhou B, Zhang S, Zhu Y, Han L, Geng Y, Jin Z, Liu H, Wang D, Zhao Y, Wen Y, Cui W, Zhou Y, Gu Q, Sun C, Lu X, Wang W, Wang Y, Li C, Wang Y, Yao W, Liu P (2013) Clinical features and factors associated with severity and fatality among patients with severe fever with thrombocytopenia syndrome Bunyavirus infection in Northeast China. PloS one 8:e80802. https://doi.org/10.1371/journal.pone.0080802
Ferrari S, Toniolo A, Monaco S, Luciani F, Cainelli F, Baj A et al (2009) Viral encephalitis: etiology, clinical features, diagnosis and management. Open Infect Dis J 3:1–12
Kaneko M, Azuma T, Yasukawa M, Shinomiya H (2015) A fatal case of severe fever with thrombocytopenia syndrome complicated by hemophagocytic lymphohistiocytosis. J Jpn Assoc Infect Dis (Kansenshogaku Zasshi) 89:592e6. 26630792
Li DX (2015) Severe fever with thrombocytopenia syndrome: a newly discovered emerging infectious disease. Clin Microbiol Infect. 21(7):614–620. https://doi.org/10.1016/j.cmi.2015.03.001
Liu Q, He B, Huang SY, Wei F, Zhu XQ (2014) Severe fever with thrombocytopenia syndrome, an emerging tick-borne zoonosis. Lancet Infect Dis 14:763–772. https://doi.org/10.1016/S1473-3099(14)70718-2
Overby AK, Pettersson RF, Neve EP (2007) The glycoprotein cytoplasmic tail of Uukuniemi virus (Phenuiviridae) interacts with ribonucleoproteins and is critical for genome packaging. J. Virol. 81:3198–3205. https://doi.org/10.1128/JVI.02655-06
Papa MP, Meuren LM, Coelho SVA, Lucas CGO, Mustafa YM, Lemos Matassoli F et al (2017) Zika Virus infects, activates, and crosses brain microvascular endothelial cells, without barrier disruption. Front Microbiol 22(8):2557. https://doi.org/10.3389/fmicb.2017.02557
Park SY, Kwon JS, Kim JY, Kim SM, Jiang YR, Kim MC et al (2018) Severe fever with thrombocytopenia syndrome-associated encephalopathy/encephalitis. Clin Microbiol Infect 24(4):432–e1-432. https://doi.org/10.1016/j.cmi.2017.09.002
Pettersson RF, Melin L (1996) Synthesis, assembly, and intracellular transport of Phenuiviridae membrane proteins. In: Elliott R (ed) The Phenuiviridae. Plenum Press, New York, USA, pp 159–183
Strandin T, Hepojoki J, Vaheri A (2013) Cytoplasmic tails of bunyavirus Gn glycoproteins-could they act as matrix protein surrogates? Virology. 437:73–80. https://doi.org/10.1016/j.virol.2013.01.001
Xie YH, Yang JY, Tan Y, Lin M, Bi FY, Wu XH et al (2012) Clinical features and evaluation of clinical diagnosis of viral encephalitis. Dis Surveill 27:256–262
Xu B, Liu L, Huang X, Ma H, Zhang Y, Du Y et al (2011) Metagenomic analysis of fever, thrombocytopenia and leukopenia syndrome (FTLS) in Henan Province, China: Discovery of a New Bunyavirus. PLoS Pathog 7(11):e1002369. https://doi.org/10.1371/journal.ppat.1002369
Yu XJ, Liang MF, Zhang SY, Liu Y, Li JD, Sun YL et al (2011) Fever with thrombocytopenia associated with a novel bunyavirus in China. N Engl J Med 364:1523–1532. https://doi.org/10.1056/NEJMoa1010095
Yuan F, Zheng A (2017 Feb) Entry of severe fever with thrombocytopenia syndrome virus. Virol Sin. 32(1):44–50. https://doi.org/10.1007/s12250-016-3858-6
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Wang, C., Gong, L., Zeng, Z. et al. Genome-based analysis of SFTSV causing severe encephalitis with brain lesions. J. Neurovirol. 26, 181–187 (2020). https://doi.org/10.1007/s13365-019-00816-0
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DOI: https://doi.org/10.1007/s13365-019-00816-0