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Lyssavirus phosphoproteins increase mitochondrial complex I activity and levels of reactive oxygen species

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Abstract

We have previously demonstrated that serine residues at positions 162 and 166 of the rabies virus (RABV) phosphoprotein (P) are critical for oxidative stress induced by CVS in cultured cells. We have now evaluated the P of two street RABV variants and Mokola (MOK) virus. The P of these viruses, like CVS, induces an increase in complex I activities and reactive oxygen species levels in transfected cells. Although the sequence homology of P is only 45% with MOK (higher for street viruses) and CVS, serine residues are conserved at positions 162 and 166, suggesting their potential importance in oxidative stress.

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Acknowledgments

We wish to acknowledge Stephanie Booth and Anna Majer (Public Health Agency of Canada, Winnipeg, Manitoba, Canada) for assistance with the microarrays.

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Authors and Affiliations

  1. Department of Internal Medicine (Neurology), University of Manitoba, GF-543, 820 Sherbrook Street, Winnipeg, MB, R3A 1R9, Canada

    Wafa Kammouni & Alan C. Jackson

  2. Zoonotic Diseases and Special Pathogens, Public Health Agency of Canada, Winnipeg, MB, Canada

    Heidi Wood

  3. Department of Medical Microbiology, University of Manitoba, GF-543, 820 Sherbrook Street, Winnipeg, MB, R3A 1R9, Canada

    Heidi Wood & Alan C. Jackson

Authors
  1. Wafa Kammouni
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  2. Heidi Wood
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  3. Alan C. Jackson
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Correspondence to Alan C. Jackson.

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Funding statement

This work was supported by a Research Manitoba Bridge Funding Award and the Department of Internal Medicine, University of Manitoba (to A.C. Jackson).

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The authors declare that they have no conflict of interest.

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Kammouni, W., Wood, H. & Jackson, A.C. Lyssavirus phosphoproteins increase mitochondrial complex I activity and levels of reactive oxygen species. J. Neurovirol. 23, 756–762 (2017). https://doi.org/10.1007/s13365-017-0550-z

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  • DOI: https://doi.org/10.1007/s13365-017-0550-z

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