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JC virus nucleotides 376-396 are critical for VP1 capsid protein expression

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Abstract

JC virus (JCV) infection of the brain can cause progressive multifocal leukoencephalopathy, JCV granule cell neuronopathy, and JCV encephalopathy (JCVE). JCVCPN, isolated from the brain of a patient with JCVE, is a naturally occurring strain of JCV with a 143-base pair deletion in the agnogene. Cell culture studies of JCVCPN have shown that the loss of these nucleotides in the agnogene results in impaired expression of VP1 and infectious virion production. To better understand the role of this DNA sequence in JCV replication, we generated a series of deletions in the agnogene on the backbone of a virus which has a mutated agnoprotein start codon preventing agnoprotein expression. We found that deletion of nucleotides 376-396 results in decreased levels of viral DNA replication and a lack of VP1 expression. These results indicate that these nucleotides play a crucial role in JCV replication.

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Acknowledgments

This work was supported in part by NIH R01 NS 074995 to IJK.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Division of Neurovirology, Department of Neurology, Beth Israel Deaconess Medical Center, Harvard Medical School, E/CLS-1005, 330 Brookline Avenue, Boston, MA, 02215, USA

    Laura C. Ellis & Igor J. Koralnik

  2. Center for Virology and Vaccine Research, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA

    Laura C. Ellis & Igor J. Koralnik

  3. Harvard Program in Virology, Harvard Medical School, Boston, MA, USA

    Laura C. Ellis

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  1. Laura C. Ellis
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  2. Igor J. Koralnik
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Correspondence to Igor J. Koralnik.

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Ellis, L.C., Koralnik, I.J. JC virus nucleotides 376-396 are critical for VP1 capsid protein expression. J. Neurovirol. 21, 671–678 (2015). https://doi.org/10.1007/s13365-014-0278-y

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  • DOI: https://doi.org/10.1007/s13365-014-0278-y

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