Abstract
Human parechoviruses (HPeV) are classified into 14 genotypes. HPeV1 and HPeV2 are the most prevalent genotypes in young children, which have been associated with mild to severe diseases. This study was conducted to investigate the involvement of HPeVs in aseptic meningitis and sepsis-like illness in Iran. Viral RNA was extracted from 148 cerebrospinal fluid samples from children <8 years old with primary diagnosis of aseptic meningitis and/or sepsis-like illness. Specific HPeV, HEV real-time PCR and HPeV typing were done to identify the infection rate of these viruses. HPeV and HEV were detected in 64 (43.24 %), 31 (20.94 %) of 148 patients with 10 (6.75 %) coinfection. VP1/VP3 junction region was successfully sequenced from 12 of the HPeV-positive specimens, and all of them were identified as HPeV1. HPeV was more prevalent than HEV in both aseptic meningitis and sepsis-like illness, so further studies are needed to understand the disease burden of HPeV infections, and clinical manifestations especially in specific illnesses of possible viral etiology. Direct detection of these viruses leads to reduce hospitalization and use of antibiotic, which are often followed by other complications in neonates and young children.
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Acknowledgments
We would like to greatly appreciate Professor Glyn Stanway (Department of Biological Sciences, University of Essex, UK) for kindly providing us with the positive control of HPeV1. We also wish to thank Professor W. Allan Nix (Centers for Disease Control and Prevention, Atlanta, USA) and Dr. Kimberley Benschop (National Institute of Public Health and the Environment, The Netherlands) for their great help and advice on this study. We thank Dr. K. Azadmanesh (Head of Department of Virology, Pasteur Institute of Iran), and Mrs. Z. Shahosseini (Department of Virology, Pasteur Institute of Iran) for their contribution to this study.
Funding
This project was supported by the Pasteur Institute of Iran under the project number 526.
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None declared.
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Rahimi, P., Naser, H.M., Siadat, S.D. et al. Genotyping of human parechoviruses in Iranian young children with aseptic meningitis and sepsis-like illness. J. Neurovirol. 19, 595–600 (2013). https://doi.org/10.1007/s13365-013-0221-7
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DOI: https://doi.org/10.1007/s13365-013-0221-7