Abstract
An examination was made of fluoride content in the mandibular first molars of the permanent teeth of the red fox Vulpes vulpes living in north-west (NW) Poland. The teeth were first dried to a constant weight at 105°C and then ashed. Fluorides were determined potentiometrically, and their concentrations were expressed in dry weight (DW) and ash. The results were used to perform an indirect estimation of fluoride pollution in the examined region of Poland. The collected specimens (n = 35) were classified into one of the three age categories: immature (im, 6–12 months), subadult (subad, from 12 to 20 months) and adult (ad, >20 months). The mean concentrations (geometric mean) of fluoride were similar in the im and subad groups (230 and 296 mg/kg DW and 297 and 385 mg/kg ash, respectively), and significantly smaller than in the ad group (504 and 654 mg/kg, respectively, in DW and ash). Basing on other reports that the ∼400 mg/kg DW concentration of fluoride in bones in the long-lived wild mammals generally reflects the geochemical background, it was found that 57% of the foxes in NW Poland exceeded this value by 9% to 170%. This indirectly reflects a moderate fluoride contamination in the tested region.
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References
Bassin EB, Wypij D, Davis RB, Mittelman MA (2006) Age-specific fluoride exposure in drinking water and osteosarcoma (United States). Cancer Causes Control 17:421–428. doi:10.1007/s10552-005-0500-6
Bezerra de Menezes LM, Volpato MC, Rosalen PL, Cury JA (2003) Bone as a biomarker of acute fluoride toxicity. Forensic Sci Inter 137:209–214. doi:10.1016/j.forsciint.2003.08.001
Boulton IC, Cooke JA, Johnson MS (1999) Lesion scoring in field vole teeth: application to the biological monitoring of environmental fluoride contamination. Environ Monit Asses 55:409–422. doi:10.1023/A:1005987016084
Camargo JA (2003) Fluoride toxicity to aquatic organisms: a review. Chemosphere 50:251–264. doi:10.1016/S0045-6535(02)00498-8
Choubisa SL (2008) Dental fluorosis in domestic animals. Curr Sci 95:1674–1675
Churcher CS (1960) Cranial variation in the North American red fox. J Mammal 4:349–360
Corsolini S, Focardi S, Leonzio C, Lovari S, Monaci F, Romeo G (1999) Heavy metals and chlorinated hydrocarbon concentrations in the red fox in relation to some biological parameters. Environ Monit Assess 54:87–100
Dabkowska E, Machoy-Mokrzyńska A, Straszko J, Machoy Z, Samujło D (1995) Temporal changes in the fluoride levels of jaws of European deer in industrial regions of Western Pomerania, Poland. Environ Geochem Health 17:155–158
Den Besten PK (1994) Dental fluorosis: its use as a biomarker. Adv Dent Res 8:105–110. doi:10.1177/08959374940080010201
Dip R, Stieger C, Deplazes P, Hegglin D, Mueller U, Dafflon O, Koch H (2001) Comparison of heavy metal concentration in tissues of red foxes from suburban and rural areas. Arch Environ Contam Toxicol 40:551–556. doi:10.1007/s002440010209
Eckerlin EH, Maylin GA, Krook L, Carmichael D (1988) Ameliorative effects of reduced food-borne fluoride on reproduction in silver foxes. Cornell Vet 78:385–391
Ellenberg H (1991) Bioindicator and biological monitoring. In: Biological monitoring. Signal from the environment. Gate/GTZ, Braunschweig, pp 13–74
ES WV (2009) Environmental state in West Pomerania Voivodship in 2008 [Stan środowiska w województwie zachodniopomorskim w 2008 roku]. www.wios.szczecin.pl [in Polish]
Gołdyn B, Hromada MA, Surmacki A, Tryjanowski P (2003) Habitat use and diet of the red fox Vulpes vulpes in an agricultural landscape in Poland. Zeitschr Jagwis 49:191–200
Gutiérrez-Salazar MP, Reyes-Gasga J (2003) Microhardness and chemical composition of human tooth. Mater Res 6:367–373. doi:10.1590/S1516-14392003000300011
Gutowska I, Machoy Z, Machaliński B, Chlubek D (2004) Living conditions of deer in the provinces of Western Pomerania and Lubuskie as revealed by mandibular content of fluoride, calcium, and magnesium. 1. Inter-relations between fluoride, calcium, and magnesium content in mandible. Ann Acad Med Stet (suppl) 50:42–46, in Polish, English summary
Harrison PTC (2005) Fluoride in water: a UK perspective. J Fluor Chem 126:1448–1456. doi:10.1016/j.jfluchem.2005.09.009
Inkielewicz I, Czarnowski W, Krechniak J (2003) Determination of fluoride in soft tissues. Fluoride 36:16–20
Inorganic Fluorides—PSL1 (1993) Canadian Environmental Protection Act. Environment Canada Health, Canada (www.hc-sc.gc.ca/ewh-semt/pubs/contaminants/psl1)
Jelenko I, Pokorny B (2010) Historical biomonitoring of fluoride pollution by determining fluoride contents in roe deer (Capreolus capreolus L.) antlers and mandibles in the vicinity of the largest Slovene thermal power plant. Sci Total Environ 409:430–438. doi:10.1016/j.scitotenv.2010.10.012
Kalisińska E, Lisowski P, Salicki W, Kucharska T, Kavetska K (2009) Mercury in wild terrestrial carnivorous mammals from north-western Poland and unusual fish diet of red fox. Acta Theriol 54:345–356. doi:10.4098/j.at.0001-7051.032.2008
Kalisińska E, Palczewska M (2007) Fluoride in the teeth of red fox Vulpes vulpes from western Pomerania. Ochr Środ Zas Nat 31:428–433, in Polish, English summary
Kay CE, Tourangeau PC, Gordon CC (1975) Fluoride levels in indigenous animals and plants collected from uncontaminated ecosystems. Fluoride 8:125–133
Kierdorf U, Kierdorf H, Sedlacek F, Fejerskov O (1996) Structural changes in fluorosed dental enamel of reed deer (Cervus elaphus L.) from a region serve environmental pollution by fluorides. J Anat 188:183–195
Kierdorf H, Kierdorf U, Richards A, Sedlacek F (2000) Disturbed enamel formation in wild boars (Sus scrofa L.) from fluoride polluted areas in Central Europe. Anat Rec 259:12–24. doi:https://doi.org/10.1002/(SICI)1097-0185(20000501)259:1<12::AID-AR2>3.0.CO;2-6
Li DS, Cutress TW, Pearce EIF, Coote GE (1996) Effects of arsenic or/and fluoride on mineralized tissues of the rat. Fluoride 29:156–162
Macdonald DW, Reynolds JC (2004) Red fox Vulpes vulpes Linnaeus, 1758. In: Sillero-Zubiri C, Hoffmann M, Macdonald DW (eds) Canids: foxes, wolves, jackals and dogs. Status survey and conservation action plan. IUCN/SSC Canid Specialist Group. Gland, Switzerland and Cambridge, pp 129–136
Machoy Z, Dabkowska D, Samujło D, Ogoński T, Raczyński J, Gębczyńska Z (1995) Relationships between fluoride content in bones and the age in European elk (Alces alces L.). Comp Biochem Physiol 3C:117–120
Macicek P, Krook LP (2008) Fluorosis in horses drinking artificially fluoridated water. Fluoride 41:177–183
Ozsvath DL (2009) Fluoride and environmental health: a review. Rev Environ Sci Biotech 8:59–79. doi:10.1007/s11157-008-9136-9
Qin C, Brunn JC, Jones J, George A, Ramachandran A, Gorski JP, Butler WT (2001) A comparative study of sialic acid-rich proteins in rat bone and dentin. Eur J Oral Sci 109:133–141. doi:10.1034/j.1600-0722.2001.00001.x
Richter H, Kierdorf U, Richards A, Kierdorf H (2010) Dentin abnormalities in cheek teeth of wild red deer and roe deer from a fluoride-polluted area in Central Europe. Ann Anat 192:86–95. doi:10.1016/j.aanat.2009.12.003
Saiani RA, Porto IM, Marcantonio E, Cury JA, de Sousa FB, Gerlach RF (2009) Morphological characterisation of rat incisor fluorotic lesions. Arch Oral Biol 54:1008–1015. doi:10.1016/j.archoralbio.2009.08.009
Sidorovich VE, Sidorovich A, Izotova IV (2006) Variations in the diet and population density of the red fox Vulpes vulpes in the mixed woodlands on northern Belarus. Mamm Biol 71:74–89
Suttie JS, Dickie R, Clay AB, Nielsen P, Mahan WE, Baumann DP, Hamilton RJ (1987) Effects of fluoride emissions from modern primary aluminium smelter on a local population of white-tailed deer (Odocoileus virginianus). J Wildl Dis 23:135–143
Tataruch F, Kierdorf H (2004) Mammals as biomornitors. In: Markert AA, Breure AM, Zechmeister HG (eds) Bioindicators and biomonitors. Elsevier, Amsterdam, pp 737–772
Telesiński A, Śnioszek M (2009) Bioindicators of environmental pollution with fluorine. Bromatol Chem Toksykol 42:1148–1154, in Polish, English summary
Verkerk RHJ (2010) The paradox of overlapping micronutrient risks and benefits obligates risk/benefit analysis. Toxicology 278:27–38. doi:10.1016/j.tox.2010.02.011
Vieira AP, Mousny M, Maia R, Hancock R, Everett ET, Grynpas MD (2005) Assessment of teeth as biomarkers for skeletal fluoride exposure. Osteoporos Int 16:1576–1582. doi:10.1007/s00198-005-1870-z
Vieira AP, Hancock R, Maia R, Limeback H, Grynpas MD (2004) Is fluoride concentration in dentin and enamel a good indicator of dental fluorosis? J Dent Res 83:76–80. doi:10.1177/154405910408300115
Walton KC (1984) Fluoride in fox bone near an aluminium reduction plant in Anglesy, Wales, and elsewhere in the United Kingdom. Environ Pollut B 7:273–280
Walton KC (1988) Environmental fluoride and fluorosis in mammals. Mamm Rev 18:77–90. doi:10.1111/j.1365-2907.1988.tb00077.x
Weinstein LH, Davison A (2004) Fluorides in the environment. CABI Publishing, Oxford
Zakrzewska H (1996) Evaluation of some biochemical parameters in blood serum and bones of sheep Western Pomerania regions of various contamination degree. Ann Acad Med Stet 10:105–125
Zakrzewska H, Machoy-Mokrzyńska A, Materny M, Gutowska I, Machoy Z (2005) Estimation of fluoride distribution in the mandible and teeth of the red deer (Cervus elaphus L.) from industrially polluted areas in Poland. Arch Oral Biol 50:309–316. doi:10.1016/j.archoralbio.2004.08.012
Zemek F, Herman M, Kierdorf H, Kierdorf U, Sedlácek F (2006) Spatial distribution of dental fluorosis in roe deer (Capreolus capreolus) from North Bohemia (Czech Republic) and its relationships with environmental factors. Sci Total Environ 370:491–505. doi:10.1016/j.scitotenv.2006.04.027
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Communicated by: Mathew W. Hayward
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Kalisińska, E., Palczewska-Komsa, M. Teeth of the red fox Vulpes vulpes (L., 1758) as a bioindicator in studies on fluoride pollution. Acta Theriol 56, 343–351 (2011). https://doi.org/10.1007/s13364-011-0039-8
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DOI: https://doi.org/10.1007/s13364-011-0039-8