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Characterization of Isomeric Glycans by Reversed Phase Liquid Chromatography-Electronic Excitation Dissociation Tandem Mass Spectrometry

  • Yang Tang
  • Juan Wei
  • Catherine E. Costello
  • Cheng Lin
Focus: Mass Spectrometry in Glycobiology and Related Fields: Research Article

Abstract

The occurrence of numerous structural isomers in glycans from biological sources presents a severe challenge for structural glycomics. The subtle differences among isomeric structures demand analytical methods that can provide structural details while working efficiently with on-line glycan separation methods. Although liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a powerful tool for mixture analysis, the commonly utilized collision-induced dissociation (CID) method often does not generate a sufficient number of fragments at the MS2 level for comprehensive structural characterization. Here, we studied the electronic excitation dissociation (EED) behaviors of metal-adducted, permethylated glycans, and identified key spectral features that could facilitate both topology and linkage determinations. We developed an EED-based, nanoscale, reversed phase (RP)LC-MS/MS platform, and demonstrated its ability to achieve complete structural elucidation of up to five structural isomers in a single LC-MS/MS analysis.

Graphical Abstract

Keywords

Isomer analysis Glycan FTICR MS Electronic excitation dissociation Reversed phase liquid chromatography 

Notes

Funding Information

This research is supported by NIH grants P41 GM104603, R21 GM122635, U01 CA221234, and S10 RR025082.

Supplementary material

13361_2018_1943_MOESM1_ESM.docx (320 kb)
ESM 1 (DOCX 319 kb)

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© American Society for Mass Spectrometry 2018

Authors and Affiliations

  1. 1.Department of ChemistryBoston UniversityBostonUSA
  2. 2.Center for Biomedical Mass SpectrometryBoston University School of MedicineBostonUSA

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