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Direct Analysis in Real Time-Mass Spectrometry for the Rapid Detection of Metabolites of Aconite Alkaloids in Intestinal Bacteria

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Journal of The American Society for Mass Spectrometry

Abstract

In the present work, direct analysis of real time ionization combined with multi-stage tandem mass spectrometry (DART-MSn) was used to investigate the metabolic profile of aconite alkaloids in rat intestinal bacteria. A total of 36 metabolites from three aconite alkaloids were identified by using DART-MSn, and the feasibility of quantitative analysis of these analytes was examined. Key parameters of the DART ion source, such as helium gas temperature and pressure, the source-to-MS distance, and the speed of the autosampler, were optimized to achieve high sensitivity, enhance reproducibility, and reduce the occurrence of fragmentation. The instrument analysis time for one sample can be less than 10 s for this method. Compared with ESI-MS and UPLC-MS, the DART-MS is more efficient for directly detecting metabolic samples, and has the advantage of being a simple, high-speed, high-throughput method.

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References

  1. Zheng, X.J., Xie, G.X., Zhao, A.H., Zhao, L.J., Yao, C., Chiu, N.H.L., Zhou, Z.X., Bao, Y.Q., Jia, W.P., Nicholson, J.K., Jia, W.: The footprints of gut microbial-mammalian co-metabolism. J. Proteome Res. 10, 5512–5522 (2011)

    Article  CAS  Google Scholar 

  2. Wang, X.Y., Pi, Z.F., Liu, W.L., Zhao, Y.F., Liu, S.Y.: Effect of pH on the metabolism of aconitine under rat intestinal bacteria and analysis of metabolites using HPLCMS-MSn technique. Chin. J. Chem. 28, 2494–2500 (2010)

    Article  CAS  Google Scholar 

  3. Xin, Y., Pi, Z.F., Song, F.R., Liu, Z.Q., Liu, S.Y.: Study on the metabolic characteristics of aconite alkaloids in the extract of Radix aconiti under intestinal bacteria of rat by UPLC/MSn technique. Chin. J. Chem. 30, 656–664 (2012)

    Article  CAS  Google Scholar 

  4. Kawata, Y., Ma, C.-M., Meselhy, M.R., Nakamura, N., Wang, H., Hattori, M., Namba, T., Satoh, K., Kuraishi, Y.: Conversion of aconitine to lipoaconitine by human intestinal bacteria and their antinociceptive effects in mice. J. Trad. Med. 16, 15–23 (1999)

    CAS  Google Scholar 

  5. Rajchl, A., Drgova, L., Gregrova, A., Cizkova, H., Sevcik, R., Voldrich, M.: Rapid determination of 5-hydroxymethylfurfural by DART ionization with time-of-flight mass spectrometry. Anal. Bioanal. Chem. 405, 4737–4745 (2013)

    Article  CAS  Google Scholar 

  6. Zhou, Z.G., Zhang, J.L., Zhang, W., Bai, Y., Liu, H.W.: Rapid screening for synthetic antidiabetic drug adulteration in herbal dietary supplements using direct analysis in real time mass spectrometry. Analyst 136, 2613–2618 (2011)

    Article  CAS  Google Scholar 

  7. Zhou, M., McDonald, J.F., Fernandez, F.M.: Optimization of a direct analysis in real time/time-of-flight mass spectrometry method for rapid serum metabolomic fingerprinting. J. Am. Soc. Mass Spectrom. 21, 68–75 (2010)

    Article  Google Scholar 

  8. Jagerdeo, E., Abdel-Rehim, M.: Screening of cocaine and its metabolites in human urine samples by direct analysis in real-time source coupled to time-of-flight mass spectrometry after online preconcentration utilizing microextraction by packed sorbent. J. Am. Soc. Mass Spectrom. 20, 891–899 (2009)

    Article  CAS  Google Scholar 

  9. Zhu, H.B., Wang, C.Y., Qi, Y., Song, F.R., Liu, Z.Q., Liu, S.Y.: Rapid quality assessment of Radix aconiti preparata using direct analysis in real time (DART) mass spectrometry. Anal. Chim. Acta. 752, 69–77 (2012)

    Article  CAS  Google Scholar 

  10. Wang, X.Y., Pi, Z.F., Liu, W.L., Song, F.R., Liu, Z.Q., Liu, S.Y.: Biotransformation of aconitum alkaloids before and after the combination of Radix aconiti preparata by rat intestinal flora using semiquantitative analysis method of electrospray ionization mass spectrometry. Chem. J. Chin. Univ. 32, 1526–1531 (2011)

    CAS  Google Scholar 

Download references

Acknowledgments

This research work was supported by grants from the National Natural Science Foundation of China (no. 81073040, no. 81274046) and National Basic Research Program of China (973 Program) (2011CB505300, 2011CB505305).

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Authors and Affiliations

  1. Chemical Biology Laboratory and Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China

    Xue Li, Guangyue Hou, Junpeng Xing, Fengrui Song, Zhiqiang Liu & Shuying Liu

  2. University of the Chinese Academy of Sciences, Beijing, 100039, China

    Xue Li & Guangyue Hou

Authors
  1. Xue Li
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  2. Guangyue Hou
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  3. Junpeng Xing
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  4. Fengrui Song
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  5. Zhiqiang Liu
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  6. Shuying Liu
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Corresponding author

Correspondence to Fengrui Song.

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Li, X., Hou, G., Xing, J. et al. Direct Analysis in Real Time-Mass Spectrometry for the Rapid Detection of Metabolites of Aconite Alkaloids in Intestinal Bacteria. J. Am. Soc. Mass Spectrom. 25, 2181–2184 (2014). https://doi.org/10.1007/s13361-014-0991-z

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  • DOI: https://doi.org/10.1007/s13361-014-0991-z

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