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Improved MALDI-TOF Microbial Mass Spectrometry Imaging by Application of a Dispersed Solid Matrix

  • Application Note
  • Published:
Journal of The American Society for Mass Spectrometry

Abstract

The key step in high quality microbial matrix-assisted laser desorption/ionization mass spectrometry imaging (microbial MALDI MSI) is the fabrication of a homogeneous matrix coating showing a fine-grained morphology. This application note addresses a novel method to apply solid MALDI matrices onto microbial cultures grown on thin agar media. A suspension of a mixture of 2,5-DHB and α-CHCA is sprayed onto the agar sample surface to form highly homogeneous matrix coatings. As a result, the signal intensities of metabolites secreted by the fungus Aspergillus fumigatus were found to be clearly enhanced.

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Acknowledgments

The authors thank the Austrian Ministry of Science (Sparkling Science Project SPA04/144 to T.M.) and the Austrian Science Foundation FWF (ERA-NET/PathoGenoMics project TRANSPAT I282-B09 to H.H.) for financial support, and the University of Applied Sciences Tyrol (FHG Innsbruck) for access to the fluorescence microscopy equipment.

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Authors and Affiliations

  1. Institute of Organic Chemistry, University of Innsbruck, 6020, Innsbruck, Austria

    Stefan Vergeiner & Thomas Müller

  2. Division of Molecular Biology, Innsbruck Medical University, 6020, Innsbruck, Austria

    Lukas Schafferer & Hubertus Haas

Authors
  1. Stefan Vergeiner
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  2. Lukas Schafferer
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  3. Hubertus Haas
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  4. Thomas Müller
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Corresponding authors

Correspondence to Hubertus Haas or Thomas Müller.

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Vergeiner, S., Schafferer, L., Haas, H. et al. Improved MALDI-TOF Microbial Mass Spectrometry Imaging by Application of a Dispersed Solid Matrix. J. Am. Soc. Mass Spectrom. 25, 1498–1501 (2014). https://doi.org/10.1007/s13361-014-0923-y

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  • DOI: https://doi.org/10.1007/s13361-014-0923-y

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