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Spectral Reproducibility and Quantification of Peptides in MALDI of Samples Prepared by Micro-Spotting

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Journal of The American Society for Mass Spectrometry

Abstract

Previously, we reported that MALDI spectra of peptides became reproducible when temperature was kept constant. Linear calibration curves derived from such spectral data could be used for quantification. Homogeneity of samples was one of the requirements. Among the three popular matrices used in peptide MALDI [i.e., α-cyano-4-hydroxycinnamic acid (CHCA), 2,5-dihydroxybenzoic acid (DHB), and sinapinic acid (SA)], homogeneous samples could be prepared by conventional means only for CHCA. In this work, we showed that sample preparation by micro-spotting improved the homogeneity for all three cases.

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Acknowledgments

The authors acknowledge support for this work by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MEST) (2013076408). K.M.P. and S.H.A. thank the Ministry of Education, Science, and Technology, Republic of Korea, for Brain Korea 21 Fellowship.

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Authors and Affiliations

  1. Department of Chemistry, Seoul National University, Seoul, 151-747, Korea

    Yong Jin Bae, Kyung Man Park, Sung Hee Ahn & Myung Soo Kim

  2. Medical Proteomics Research Center, KRIBB, Daejeon, 305-806, Korea

    Jeong Hee Moon

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  1. Yong Jin Bae
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  2. Kyung Man Park
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  3. Sung Hee Ahn
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Correspondence to Myung Soo Kim.

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Bae, Y.J., Park, K.M., Ahn, S.H. et al. Spectral Reproducibility and Quantification of Peptides in MALDI of Samples Prepared by Micro-Spotting. J. Am. Soc. Mass Spectrom. 25, 1502–1505 (2014). https://doi.org/10.1007/s13361-014-0919-7

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  • DOI: https://doi.org/10.1007/s13361-014-0919-7

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