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Activation Energies of Fragmentations of Disaccharides by Tandem Mass Spectrometry

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Journal of The American Society for Mass Spectrometry


A simple multiple collision model for collision induced dissociation (CID) in quadrupole was applied for the estimation of the activation energy (Eo) of the fragmentation processes for lithiated and trifluoroacetated disaccharides, such as maltose, cellobiose, isomaltose, gentiobiose, and trehalose. The internal energy-dependent rate constants k(Eint) were calculated using the Rice-Ramsperger-Kassel-Marcus (RRKM) or the Rice-Ramsperger-Kassel (RRK) theory. The Eo values were estimated by fitting the calculated survival yield (SY) curves to the experimental ones. The calculated Eo values of the fragmentation processes for lithiated disaccharides were in the range of 1.4–1.7 eV, and were found to increase in the order trehalose < maltose < isomaltose < cellobiose < gentiobiose.

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The authors acknowledge financial support for this work by the grant K-101850 given by OTKA (National Found for Scientific Research Development, Hungary), and the grant TÁMOP-4.2.2.A-11/1/KONV-2012-0036 by the European Union and co-funded by the European Social Fund.

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Correspondence to Sándor Kéki.

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Kuki, Á., Nagy, L., Szabó, K.E. et al. Activation Energies of Fragmentations of Disaccharides by Tandem Mass Spectrometry. J. Am. Soc. Mass Spectrom. 25, 439–443 (2014).

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