Advantages of Monodisperse and Chemically Robust “SpheriCal” Polyester Dendrimers as a “Universal” MS Calibrant

Abstract

The utilization of dendrimer calibrants as an alternative to peptides and proteins for high mass calibration is explored. These synthetic macromolecules exhibited a number of attractive advantages, including exceptional shelf-lives, broad compatibility with a wide range of matrices and solvents, and evenly spaced calibration masses across the mass range examined, 700–30,000 u. The exceptional purity of these dendrimers and the technical simplicity of this calibration platform validate their broad relevance for high molecular weight mass spectrometry.

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Acknowledgments

This work was performed with the help of Tulane University and the Swedish Governmental Agency for Innovation Systems, VINNOVA, through the Forska&Väx program.

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Correspondence to Scott M. Grayson.

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Grayson, S.M., Myers, B.K., Bengtsson, J. et al. Advantages of Monodisperse and Chemically Robust “SpheriCal” Polyester Dendrimers as a “Universal” MS Calibrant. J. Am. Soc. Mass Spectrom. 25, 303–309 (2014). https://doi.org/10.1007/s13361-013-0777-8

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Key words

  • Calibrant
  • Dendrimer
  • Calibration
  • Polymer
  • MALDI-TOF MS