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157 nm Photodissociation of Dipeptide Ions Containing N-Terminal Arginine

  • Research Article
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Journal of The American Society for Mass Spectrometry

Abstract

Twenty singly-charged dipeptide ions with N-terminal arginine were photodissociated using 157 nm light in both a linear ion-trap mass spectrometer and a MALDI-TOF-TOF mass spectrometer. Analogous to previous work on dipeptides containing C-terminal arginine, this set of samples enabled insights into the photofragmentation propensities associated with individual residues. In addition to familiar products such as a-, d-, and immonium ions, m2 and m2+13 ions were also observed. Certain side chains tended to cleave between their β and γ carbons without necessarily forming d- or w-type ions, and a few other ions were produced by the high-energy fragmentation of multiple bonds.

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Acknowledgments

The authors acknowledge support for this work by grant CHE-1012855 from the National Science Foundation.

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  1. Department of Chemistry, Indiana University, Bloomington, IN, 47405, USA

    Nathaniel Webber, Yi He & James P. Reilly

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  1. Nathaniel Webber
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  2. Yi He
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  3. James P. Reilly
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Correspondence to James P. Reilly.

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Webber, N., He, Y. & Reilly, J.P. 157 nm Photodissociation of Dipeptide Ions Containing N-Terminal Arginine. J. Am. Soc. Mass Spectrom. 25, 196–203 (2014). https://doi.org/10.1007/s13361-013-0762-2

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  • DOI: https://doi.org/10.1007/s13361-013-0762-2

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