Abstract
Here we investigate the effect of S-dipalmitoylation on the electron capture dissociation (ECD) behavior of peptides. The ECD and collision induced dissociation (CID) of peptides modified by covalent attachment of [(RS)-2,3-di(palmitoyloxy)-propyl] (PAM2) group to cysteine residues [C(PAM2)LEYDTGFK and RPPGC(PAM2)SPFK] were examined. The results suggest that ECD of S-dipalmitoylated peptides can provide both primary sequence information and structural information regarding the modification. The structural information provided by CID is complementary to that provided by ECD.
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Acknowledgments
The authors gratefully acknowledge EPSRC for funding. The Thermo Fisher FT Ultra mass spectrometer used in this research was obtained through the Birmingham Science City Translational Medicine: Experimental Medicine Network of Excellence project, with support from Advantage West Midland.
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Kaczorowska, M.A., Cooper, H.J. Electron Capture Dissociation and Collision Induced Dissociation of S-Dipalmitoylated Peptides. J. Am. Soc. Mass Spectrom. 24, 1224–1227 (2013). https://doi.org/10.1007/s13361-013-0662-5
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DOI: https://doi.org/10.1007/s13361-013-0662-5