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MALDI-FTICR MS Imaging as a Powerful Tool to Identify Paenibacillus Antibiotics Involved in the Inhibition of Plant Pathogens

  • Focus: MS in the Characterization of Microorganisms: Research Article
  • Published:
Journal of The American Society for Mass Spectrometry

Abstract

Nowadays, microorganisms are more and more often used as biocontrol agents for crop protection against diseases. Among them, bacteria of Bacillus and Paenibacillus genders are already used as commercial biocontrol agents. Their mode of action is supposed to be related to their production of antibiotics, such as cyclic lipopeptides, which exhibit great antimicrobial activities. We chose to work with a Paenibacillus polymyxa strain (Pp56) very resistant to various microorganisms. The bacteria were grown simultaneously with Fusarium oxysporum and we applied matrix-assisted laser desorption/ionization-Fourier transform ion cyclotron resonance (MALDI-FTICR) mass spectrometry to identify the antibiotics compounds present in the fungus growth inhibition area. We, therefore, identified fusaricidins A, B, and C and numerous members of the LI-F antibiotics family. MALDI-FTICR mass spectrometry imaging was then used to follow the diffusion of lipopeptides involved in the inhibitory activity over time. We analyzed the molecular content of the inhibitory area at different Pp56 and Fusarium incubation durations and concluded that some lipopeptides such as fusaricidin B and a mixture of LI-F05b/06b/08a were mainly involved in the defense mechanism of Pp56. Our study confirms that MALDI imaging may be a powerful tool to quickly determine which molecular species is involved in an antagonism with another microorganism, avoiding time-consuming steps of extraction, purification, and activity tests, which are still commonly used in microbiology.

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Acknowledgments

D.D. and M.O. are post-doctoral researcher and research associate, respectively, at the FRS-FNRS (Fonds National de la Recherche Scientifique, Belgium). H.C..’s Ph.D. thesis is supported by a grant from the Fonds pour la formation à la Recherche dans l’Industrie et dans l’Agriculture (F.R.I.A.). The authors thank Laurent Franzil for technical assistance. This work received financial support from the program Fonds de la Recherche Fondamentale Collective (FRFC) n°2.4567.12 (FRS-FNRS, Belgium).

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Authors and Affiliations

  1. Mass Spectrometry Laboratory (LSM-GIGA-R), Chemistry Department, University of Liege, Liege, Belgium

    Delphine Debois & Edwin De Pauw

  2. Gembloux Agro-Bio Tech, University of Liege, Gembloux, Belgium

    Marc Ongena & Hélène Cawoy

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  1. Delphine Debois
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Correspondence to Delphine Debois.

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Debois, D., Ongena, M., Cawoy, H. et al. MALDI-FTICR MS Imaging as a Powerful Tool to Identify Paenibacillus Antibiotics Involved in the Inhibition of Plant Pathogens. J. Am. Soc. Mass Spectrom. 24, 1202–1213 (2013). https://doi.org/10.1007/s13361-013-0620-2

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