Analysis of the Formation Process of Gold Nanoparticles by Surface-Assisted Laser Desorption/Ionization Mass Spectrometry

  • Iva Tomalová
  • Chia-Hsin Lee
  • Wen-Tsen Chen
  • Cheng-Kang Chiang
  • Huan-Tsung ChangEmail author
  • Jan PreislerEmail author
Application Note


Chemical reactions of reducing agents in the gold nanoparticle (AuNP) formation process were characterized using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). As the reaction of the AuNPs progresses, the produced AuNPs can serve as an efficient SALDI substrate. SALDI-MS revealed that the reducing agents and their oxidation products can be determined in the mass spectra. With respect to the transmission electron microscopic and UV-Vis spectroscopic examination of AuNPs, SALDI-MS results confirm not only the tendency toward AuNPs formation, but also reflect the information of the redox reaction process. Our results provide useful information for developing SALDI-MS methods to explore the chemical information regarding the surface behavior between adsorbates and nanomaterials.

Key words

Nanomaterials Gold nanoparticle Surface-assisted laser desorption/ionization mass spectrometry SALDI Nanoparticle formation process 



This study was supported by the Czech Science Foundation P206/10/J012 and the Ministry of Education, Youth, and Sports of the Czech Republic CZ.1.05/1.1.00/02.0068, and the National Science Council of Taiwan under contracts NSC 101-2113-M-002-002-MY3 and NSC 99-2923-M-002-004-MY3. I.T. is supported by Brno City Municipality Scholarships for Talented Ph.D. Students.

Supplementary material

13361_2012_541_MOESM1_ESM.doc (1.1 mb)
ESM 1 (DOC 1170 kb)


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Copyright information

© American Society for Mass Spectrometry 2013

Authors and Affiliations

  • Iva Tomalová
    • 1
  • Chia-Hsin Lee
    • 2
  • Wen-Tsen Chen
    • 2
  • Cheng-Kang Chiang
    • 2
  • Huan-Tsung Chang
    • 2
    Email author
  • Jan Preisler
    • 1
    Email author
  1. 1.CEITEC MU and Department of Chemistry, Faculty of ScienceMasaryk UniversityBrnoCzech Republic
  2. 2.Department of ChemistryNational Taiwan UniversityTaipeiTaiwan

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