Abstract
The brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae) and the green rice leafhopper, Nephotettix cincticeps Uhler (Hemiptera: Cicadellidae) feed by sucking phloem sap from rice plants and excreting honeydew containing glucose. It is known that there are resistant rice cultivars against Ni. lugens or Ne. cincticeps, from which insects are unable to suck phloem sap. Here, we developed a simple method using a glucometer to measure glucose concentration in honeydew and use it to evaluate rice resistance against sucking insects. In case of Ni. lugens, the honeydew excreted by sucking a susceptible rice line was large in volume and glucose content. In contrast, the honeydew excreted by sucking a resistant rice line was low in volume and glucose content. In case of Ne. cincticeps, no glucose was detected in the honeydew excreted by sucking a resistant rice line, although more honeydew was excreted from the resistant rice line than the susceptible rice line. Therefore, it was suggested that Ne. cincticeps could not suck phloem sap of the resistant cultivar. Glucose analysis of honeydew using a glucometer would be useful for evaluating sucking inhibition of rice cultivars against Ni. lugens and Ne. cincticeps.
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Data availability
The datasets are available from the corresponding author upon reasonable request.
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Acknowledgements
We sincerely thank K. Hashino and C. Ootou of the National Agriculture and Food Research Organization for their assistance with this study. This work was partly supported by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan [Smart-breeding system for Innovative Agriculture (DIT1001)] and the Japan Society for the Promotion of Science [a Grant-in-Aid for Scientific Research (C) (JP20K06065)].
Funding
This work was supported by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan [Smart-breeding system for Innovative Agriculture (DIT1001)] and the Japan Society for the Promotion of Science [a Grant-in-Aid for Scientific Research (C) (JP20K06065)].
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YT and YH contributed to the study conception and design. Material preparation, data collection and analysis were performed by TY, YT and YM. The first draft of the manuscript was written by TY and YT and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Yokozeki, T., Matsumoto, Y., Hatakeyama, Y. et al. Evaluation of rice resistance against Nilaparvata lugens (Stål) (Hemiptera: Delphacidae) and Nephotettix cincticeps Uhler (Hemiptera: Cicadellidae) using glucometer analysis of honeydew. Appl Entomol Zool 59, 217–223 (2024). https://doi.org/10.1007/s13355-024-00865-w
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DOI: https://doi.org/10.1007/s13355-024-00865-w