Abstract
Ditylenchus destructor Thorne (Tylenchida: Anguinidae) is a plant-parasitic nematode that causes garlic rot in Japan. A method that can be used to culture the nematode on garlic has not yet been established, but is required to be able to conduct studies for a better understanding of the nematode’s physiology and ecology. The purpose of this study was to develop such a culture method. Surface-sterilized nematodes (200 individuals) were inoculated onto a surface-sterilized garlic storage leaf on 1% agar in a 100 ml medium bottle, and incubated at 25 °C. Seven weeks after inoculation, a mean number of approximately 360,000 axenic nematodes per bottle was obtained. The culture method established in this study is simple, less laborious, and can be useful for the studies of D. destructor associated with garlic.
This is a preview of subscription content, log in via an institution to check access.
Explore related subjects
Discover the latest articles and news from researchers in related subjects, suggested using machine learning.References
Block E (2010) Garlic and other Alliums. RSC Publishing, Cambridge
EPPO (2017) Ditylenchus destructor and Ditylenchus dipsaci. Bull OEPP/EPPO Bull 47:401–419. https://doi.org/10.1111/epp.12433
Fujimura T, Washio S, Nishizawa T (1986) Garlic as a new host of the potato-rot nematode, Ditylenchus destructor Thorne. Jpn J Nematol 116:38–47. https://doi.org/10.14855/jjn1972.16.38 (in Japanese with English abstract)
Fujimura T, Ichida T, Kimura T (1989) Occurrence of potato-rot nematode, Ditylenchus destructor Thorne, in garlic and control. 1. Evaluation of treatments applied before planting and after harvest for control. Jpn J Nematol 18:22–29. https://doi.org/10.14855/jjn1972.18.22 (in Japanese with English abstract)
Haraguchi S, Yoshiga T (2020) Potential of the fungal feeding nematode Aphelenchus avenae to control fungi and the plant parasitic nematode Ditylenchus destructor associated with garlic. Biol Control 143:104203. https://doi.org/10.1016/j.biocontrol.2020.104203
Kitano N, Yamashita K (2011) Effect of soil fumigant on damage of garlic caused by Ditylenchus destructor. Ann Rept Plant Prot North Jpn 62:144–147. https://doi.org/10.11455/kitanihon.2011.62_144 (in Japanese)
Nakanishi Y (1979) The host range and control of potato rot nematode, Ditylenchus destructor Thorne, in bulbous iris. Proc Kansai Pl Prot Soc 21:12–20. https://doi.org/10.4165/kapps1958.21.0_12 (in Japanese with English Summary)
Plowright RA, Caubel G, Mizen KA (2002) Ditylenchus species. In: Starr JL, Cook R, Bridge J (eds) Plant resistance to parasitic nematodes. CAB International, Wallingford, pp 107–140
Wang H, Mao J, Li R, Luo D, Zhao G, Li H (2016) A culture technique for Ditylenchus destructor on sweet potato (Ipomoea batatas). Nematology 18:501–505. https://doi.org/10.1163/15685411-00002969
Acknowledgements
The author thanks the staff of the Vegetable Research Institute, Aomori Prefectural Industrial Technology Research Center, Aomori, Japan, for providing the opportunity to begin this study. This research was partly supported by Aomori Prefectural Government.
Author information
Authors and Affiliations
Corresponding author
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic supplementary material
Below is the link to the electronic supplementary material.
13355_2020_701_MOESM1_ESM.tiff
Supplementary file1Suppl. Fig. 1 Equipment used for the aseptic Baermann funnel extraction of Ditylenchus destructor. The nematode-extraction equipment was filled with tap water and set in a 500 ml bottle, covered with aluminum foil, and autoclaved. After cooling, culture medium containing nematodes was aseptically placed in the top of the equipment on a clean bench and left overnight. Nematodes were collected in the glass tube (TIFF 2197 kb)
Rights and permissions
About this article
Cite this article
Yoshiga, T. In vitro culture method of Ditylenchus destructor (Tylenchida: Anguinidae) using garlic. Appl Entomol Zool 55, 435–437 (2020). https://doi.org/10.1007/s13355-020-00701-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13355-020-00701-x