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A stereoisomer of JHSB3 with 10S-configuration, 10S-JHSB3, biosynthesized by the corpus allatum of the brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae)

Abstract

The products released by the corpus allatum (CA), an endocrine gland producing juvenile hormone (JH), were analyzed in three species of stink bugs. Liquid chromatography–mass spectrometry (LC–MS) analysis of the CA products from female adults of Halyomorpha halys (Stål), Nezara viridula (Linnaeus), and Nezara antennata Scott (Hemiptera: Pentatomidae) revealed that the CA biosynthesized JH III skipped bisepoxyside (JHSB3) in all three species. In H. halys, in addition to JHSB3, the CA also produced its stereoisomer, 10S-JHSB3. A bioassay focusing on the number of antennal segments was adopted to examine biological activity of these products. When last instar nymphs were treated with either of the two JHs, they emerged as adults bearing nymphal type antennae with four segments in a dose-dependent fashion, indicating that both JHSB3 and 10S-JHSB3 had the JH activity in H. halys. Therefore, for the first time, 10S-JHSB3 in H. halys was found to be a naturally occurring JH molecule with 10S-configuration.

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Acknowledgements

We are grateful to Dr. H. Numata of Kyoto University for his encouragement and useful discussions, and to Dr. N. Mizutani of Kyushu Okinawa Agricultural Research Center, NARO for providing a colony of N. viridula. We thank Ms. H. Ikeda, Ms. H. Murata, Ms. U Takano and Mr. S. Enoki for rearing insects. The manuscript was read by Dr. S. G. Goto of Osaka City University, and his comments are greatly appreciated. This study was supported in part by a grant-in-aid for Scientific Research (C) (No. 1950059) to TK from the Japan Society for the Promotion of Science.

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Correspondence to Toyomi Kotaki.

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Kotaki, T., Matsumoto, K., Kaihara, K. et al. A stereoisomer of JHSB3 with 10S-configuration, 10S-JHSB3, biosynthesized by the corpus allatum of the brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae). Appl Entomol Zool 55, 223–229 (2020). https://doi.org/10.1007/s13355-020-00674-x

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  • DOI: https://doi.org/10.1007/s13355-020-00674-x

Keywords

  • Juvenile hormone
  • 10S-JHSB3
  • Halyomorpha halys
  • Nezara viridula
  • Nezara antennata