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Mitochondrial sequence polymorphism in Chinese populations of Hylobitelus xiaoi (Coleoptera: Curculionidae)

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Abstract

The Chinese weevil, Hylobitelus xiaoi Zhang, is a major pest affecting the pine industry throughout southern China, but its dispersal is still poorly understood. We aimed to investigate its dispersal by assessing the genetic structure of seven geographical populations, using the distribution of two mitochondrial genes (cytochrome c oxidase subunit I and cytochrome b). A 1212-bp fragment of the two mitochondrial genes was sequenced for 70 individuals from seven geographical populations. The gene sequences included 861 conserved sites, 351 variable sites, 254 parsim-info sites, and 97 singleton sites. These polymorphic sites defined 24 haplotypes with a haplotype diversity of 0.825. Nucleotide diversity was low (0.04568). The differentiation parameter (0.619) was much greater than the coefficient of genetic differentiation (0.285). An analysis of molecular variance suggested that most of the variation was due to within-population differences (61.68%). This molecular data also demonstrated that there is significant divergence among the seven sampled populations of H. xiaoi. This is probably due to habitat fragmentation, preventing effective gene flow between sites. Our molecular evidence supports the view that the Chinese weevil is a native pest, and that outbreaks of H. xiaoi are caused by large plantations of the exotic slash pine.

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Acknowledgements

This work was supported in part by grants from the Natural Science Foundation of JiangXi, China (Grant Nos. 20122BAB214008 and 20132BAB204024) and the National Natural Science Foundation of China (Grant No. 31460175).

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    Authors and Affiliations

    1. Water and Ecological Engineering, Nanchang Institute of Technology, Nanchang, 330099, Jiangxi, China

      Haiyan Zhang

    2. Institute of Biological Engineering, Bozhou Teachers College, Bozhou, 236800, Anhui, China

      Shengli Zhang

    3. College of Landscape and Art, Jiangxi Agriculture University, Nanchang, 330045, Jiangxi, China

      Yang Zhang, Linping Zhang, Dong Li & Fenggang Luan

    4. Jiangxi Forest Pest Control and Quarantine Bureau, Nanchang, 330038, China

      Longxi He

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    1. Haiyan Zhang
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    Correspondence to Fenggang Luan.

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    H. Zhang and S. Zhang contributed equally to this work.

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    Zhang, H., Zhang, S., Zhang, Y. et al. Mitochondrial sequence polymorphism in Chinese populations of Hylobitelus xiaoi (Coleoptera: Curculionidae). Appl Entomol Zool 52, 247–253 (2017). https://doi.org/10.1007/s13355-016-0473-9

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    • DOI: https://doi.org/10.1007/s13355-016-0473-9

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