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Sexually biased expression of odorant-binding proteins and chemosensory proteins in Asian corn borer Ostrinia furnacalis (Lepidoptera: Crambidae)

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Abstract

Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) play important roles in insect chemosensory systems. In the Asian corn borer Ostrinia furnacalis (Guenée) (Lepidoptera: Crambidae), only a fraction of them have been examined for their expression patterns. In this study, we compared the expression levels of 24 OBPs and 19 CSPs between sexes using quantitative RT-PCR. Sexually biased expression was observed for 17 OBPs and 7 CSPs in virgin individuals. Expression levels of 18 OBPs and 10 CSPs changed after mating, resulting in sexually biased expression in 16 OBPs and 1 CSP. The effect of mating was sex-specific in 9 OBPs and 2 CSPs. These results suggest a sexually divergent molecular composition and may indicate a functional divide between OBP and CSP genes in the chemosensory system of O. furnacalis.

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Correspondence to Takashi Matsuo.

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13355_2016_409_MOESM1_ESM.pdf

Fig. S1. Alignment of O. furnacalis odorant-binding proteins (OfurOBPs). Putative signal-peptide cleavage sites are marked with a vertical bar. Conserved signature cysteines are indicated by shading. Fig. S2. Alignment of O. furnacalis chemosensory proteins (OfurCSPs). Putative signal-peptide cleavage sites are marked with a vertical bar. Conserved signature cysteines are indicated by shading.(PDF 59 kb)

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Yang, B., Ozaki, K., Ishikawa, Y. et al. Sexually biased expression of odorant-binding proteins and chemosensory proteins in Asian corn borer Ostrinia furnacalis (Lepidoptera: Crambidae). Appl Entomol Zool 51, 373–383 (2016). https://doi.org/10.1007/s13355-016-0409-4

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