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Development and characterization of microsatellite loci from Metcalfa pruinosa (Hemiptera: Flatidae)

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Abstract

Metcalfa pruinosa (Say) has damaged many deciduous forest trees, ornamental trees and agricultural crops in Korea as a serious invasive alien species since 2005. It is necessary to estimate the population genetic structure and diversity for confirming the origin and invasion route to prevent successive invasions. Here, we developed and characterized eight microsatellite loci to facilitate a population genetics study of M. pruinosa. The isolated loci were polymorphic with 6–14 alleles in 40 individuals from four regional populations in Korea. The analyses revealed that they had different multilocus genotypes with the expected heterozygosity (H E) ranging from 0.491 to 0.817. Six of the eight loci did not significantly deviate from Hardy-Weinberg equilibrium. The isolated markers will be useful to trace the origin and invasion route of this invasive alien species in the future.

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References

  • Brookfield JFY (1996) A simple new method for estimating null allele frequency from heterozygote deficiency. Mol Ecol 5:453–455

    Article  CAS  PubMed  Google Scholar 

  • Duso C (1984) Infestations by Metcalfa pruinosa in the Venice district. Inf Fitopatol 34:11–14

    Google Scholar 

  • Excoffier L, Lischer HEL (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10:564–567

    Article  PubMed  Google Scholar 

  • Goudet J (1995) Fstat version 1.2: a computer program to calculate Fstatistics. J Hered 86:485–486

    Google Scholar 

  • Kijas JMH, Fowler JCS, Garbett CA, Thomas MR (1994) Enrichment of microsatellites from the citrus genome using biotinylated oligonucleotide sequences bound to streptavidin-coated magnetic particles. Biotechniques 16:656–662

    CAS  PubMed  Google Scholar 

  • Kim KS, Sappington TW (2004) Isolation and characterization of polymorphic microsatellite loci in the boll weevil, Anthonomus grandis Boheman (Coleoptera: Curculionidae). Mol Ecol Notes 4:701–703

    Article  CAS  Google Scholar 

  • Kim H, Kim M, Kwon DH, Park S, Lee Y, Jang H, Lee S, Lee SH, Huang J, Hong KJ, Jang Y (2011a) Development and characterization of 15 microsatellite loci from Lycorma delicatula (Hemiptera: Fulgoridae). Anim Cells Syst 15:295–300

    Article  Google Scholar 

  • Kim Y, Kim M, Hong KJ, Lee S (2011b) Outbreak of an exotic flatid, Metcalfa pruinosa (Say) (Hemiptera: Flatidae), in the capital region of Korea. J Asia-Pac Entomol 14:473–478

    Article  Google Scholar 

  • Kumar S, Nei M, Dudley J, Tamura K (2008) MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform 9:299–306

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Metcalf ZP, Bruner SC (1948) Cuban Flatidae with new species from adjacent regions. Ann Entomol Soc Am 41:63–97

    Article  Google Scholar 

  • Miller N, Estoup A, Toepfer S, Bourguet D, Lapchin L, Derridj S, Kim KS, Reynaud P, Furlan L, Guillemaud T (2005) Multiple transatlantic introductions of the western corn rootworm. Science 310:992

    Article  CAS  PubMed  Google Scholar 

  • Oosterhout CV, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHEKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Eco Notes 4:535–538

    Article  Google Scholar 

  • Rousset F (2008) genepop’007: a complete re-implementation of the genepop software for Windows and Linux. Mol Ecol Resour 8:103–106

    Article  PubMed  Google Scholar 

  • Rozen S, Skaletsky HJ (2000) Primer 3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386

    Google Scholar 

  • Sarno RJ, David VA, Franklin WL, O’Brien SJ, Johnson WE (2000) Development of microsatellite markers in the guanaco, Lama guanicoe: utility for South American camelids. Mol Ecol 9:1922–1924

    Article  CAS  PubMed  Google Scholar 

  • Say T (1830) Description of new North American hemipterous insects, belonging to the first family of the section Homoptera of Latreille. J Acad Nat Sci Philadelphia 6:235–244

    Google Scholar 

  • Wang XW, Trigiano RN, Windham MT, Devries RE, Scheffler BE, Rinehart TA, Spiers JM (2007) A simple PCR procedure for discovering microsatellites from small insert libraries. Mol Ecol Notes 7:558–561

    Article  CAS  Google Scholar 

  • Zangheri S, Donadini P (1980) Comparsa nel Veneto di un omottero neartico: Metcalfa pruinosa say (Homoptera, Flatidae). Redia 63:301–305

    Google Scholar 

Download references

Acknowledgments

This study was supported by a grant (project code no. 20110337127-00) from the Animal and Plant Quarantine Agency (QIA), Ministry of Food, Agriculture, Forestry and Fisheries, Republic of Korea in 2011. We thank Dr. H.S. Lee, Gyeongsangnam-do Agricultural Research and Extension Services, and Mr. Y.S. Lee, Gyeonggi-do Agricultural Research and Extension Services, for their help in supplying specimens.

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Authors and Affiliations

  1. Department of Agricultural Biotechnology, Seoul National University, 200-6102, 599 Gwanak-ro, Gwanak-gu, Seoul, 151-921, Republic of Korea

    Minyoung Kim & Seunghwan Lee

  2. Research Institute for Agriculture and Life Science, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul, 151-921, Republic of Korea

    Deok Ho Kwon & Seunghwan Lee

  3. Department of Biology, Kunsan National University, Daehangno 558, Gunsan, Jeonbuk, 573-701, Republic of Korea

    Hyojoong Kim

Authors
  1. Minyoung Kim
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  2. Deok Ho Kwon
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  3. Hyojoong Kim
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  4. Seunghwan Lee
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Corresponding author

Correspondence to Seunghwan Lee.

Additional information

Minyoung Kim and Deok Ho Kwon contributed equally to this research.

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Kim, M., Kwon, D.H., Kim, H. et al. Development and characterization of microsatellite loci from Metcalfa pruinosa (Hemiptera: Flatidae). Appl Entomol Zool 50, 131–135 (2015). https://doi.org/10.1007/s13355-014-0303-x

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  • DOI: https://doi.org/10.1007/s13355-014-0303-x

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