Abstract
We developed microsatellite markers for the fig-pollinating wasp Blastophaga nipponica Grandi using a dual-suppression-PCR technique. Twenty-one candidates of microsatellite loci were obtained, of which 15 yielded scorable patterns. The degree of polymorphism for the 15 loci was further characterized using summary statistics describing the genetic variation in 60 individuals from three natural populations in Japan. All 15 loci were polymorphic and yielded 2–27 alleles per locus. Overall observed heterozygosity (H O) and expected heterozygosity (H E) were 0.465 and 0.631, respectively. As expected, based on the inbreeding tendency of this species, the mean inbreeding coefficient (F IS) was high (= 0.255). These markers will contribute to studies on the population structure of this species.
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References
Boutin-Ganache I, Raposo M, Raymond M, Deschepper CF (2001) M13-tailed primers improve the readability and usability of microsatellite analyses performed with two different allele-sizing methods. BioTechniques 31(24–26):28
Cornille A, Underhill JG, Gruaud A, Hossaert-McKey M, Johnson SD, Tolley KA, Kjellberg F, von Noort S, Proffit M (2011) Floral volatiles, pollinator sharing and diversification in the fig-wasp mutualism: insights from Ficus natalensis, and its two wasp pollinators (South Africa). Proc R Soc B 279:1731–1739. doi:10.1098/rspb.2011.1972
Excoffier L, Lischer HEL (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10:564–567. doi:10.1111/j.1755-0998.2010.02847.x
Garcia M, Bain A, Tzeng H-Y, Peng Y-Q, Chou L-S, Kjellberg F (2012) Portable microsatellite primers for Ficus (Moraceae). Am J Bot 99:e187–e192. doi:10.3732/ajb.1100485
Goudet J (1995). FSTAT version 1.2: a computer program to calculate Fstatistics. Journal of Heredity. 86(6): 485-486. FSTAT 2.9.3.2 was available from: http://www2.unil.ch/popgen/softwares/fstat.html
Greeff JM (2002) Mating system and sex ratios of a pollinating fig wasp with dispersing males. Proc R Soc Lond B 269:2317–2323. doi:10.1098/rspb.2002.2160
Guo SW, Thompson EA (1992) Performing the exact test of Hardy–Weinberg proportion for multiple alleles. Biometrics 48:361–372. doi:10.2307/2532296
Herre E, Machado C, Bermingham E, Nason J, Windsor D, McCafferty S, Houten W, Bachmann K (1996) Molecular phylogenies of figs and their pollinator wasps. J Biogeogr 23:521–530
Jansen Van Vuuren GJ, Kryger P, Greeff JM (2006) Isolation of six microsatellite loci in the pollinating fig wasp, Platyscapa awekei. Mole Ecol Notes 6:385–386. doi:10.1111/j.1471-8286.2006.01242.x
Kerdelhue C, Le Clainche I, Rasplus J-Y (1999) Molecular phylogeny of the Ceratosolen species pollinating Ficus of the subgenus Sycomorus sensu stricto: biogeographical history and origins of the species-specificity breakdown cases. Mol Phylogenet Evol 11(3):401–414. doi:10.1006/mpev.1998.0590
Lian C, Zhou Z, Hogetsu T (2001) A simple method for developing microsatellite markers using amplified fragments of inter-simple sequence repeat (ISSR). J Plant Res 114:381–385. doi:10.1007/PL00014001
Lian C, Hogetsu T (2002) Development of microsatellite markers in black locust (Robinia pseudoacacia) using a dual-supression-PCR technique. Mol Eco Notes 2:211–213. doi:10.1046/j.1471-8286.2002.00213.x-i2
Machado CA, Jousselin E, Kjellberg F, Compton SG, Herre EA (2001) Phylogenetic relationships, historical biogeography and character evolution of fig-pollinating wasps. Proc R Soc Lond B 268:685–694. doi:10.1098/rspb.2000.1418
Molbo D, Machado C, Sevenster J, Keller L, Herre E (2003) Cryptic species of fig-pollinating wasps: Implications for the evolution of the fig-wasp mutualism, sex allocation, and precision of adaptation. Proc Natl Acad Sci USA 100:5867–5872. doi:10.1073/pnas.0930903100
Molbo D, Machado CA, Herre EA, Keller L (2004) Inbreeding and population structure in two pairs of cryptic fig wasp species. Mol Eco 13:1613–1623. doi:10.1111/j.1365-294X.2004.02158.x
Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) Micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Eco Notes 4:535–538. doi: 10.1111/j.1471-8286.2004.00684.x. http://www.microchecker.hull.ac.uk/
Oetting WS, Lee HK, Flanders DJ, Wiesner GL, Sellers TA, King RA (1995) Linkage analysis with multiplexed short tandem repeat polymorphisms using infrared fluorescence and M13 tailed primers. Genomics 30:450–458. doi:10.1006/geno.1995.1264
Su Z-H, Iino H, Nakamura K, Serrato A, Oyama K (2008) Breakdown of the one-to-one rule in Mexican fig-wasp associations inferred by molecular phylogenetic analysis. Symbiosis 45:73–81
Rousset F (2008) genepop’007: a complete re-implementation of the genepop software for Windows and Linux. Mol Eco Res 8:103–106. doi:10.1111/j.1471-8286.2007.01931.x
Rousset F, Raymond M (1995) Testing heterozygote excess and deficiency. Genetics 140:1413–1419
Weiblen G (2000) Phylogenetic relationships of functionally dioecious Ficus (Moraceae) based on ribosomal DNA sequences and morphology. Am J Bot 87:1342–1357
Wiebes J (1979) Co-evolution of figs and their insect pollinators. Annu Rev Ecol Syst 10:1–12. doi:10.1146/annurev.es.10.110179.000245
Zavodna M, Arens P, Vosman B, Van Dijk PJ, Van Damme JMM (2002) Development and characterization of microsatellite markers for Liporrhopalum tentacularis Grandi, the pollinator fig wasp of Ficus montana Blume. Mol Ecol Notes 2:569–571. doi:10.1046/j.1471-8286.2002.00319.x
Acknowledgments
This research was supported by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (no. 25650129). We thank Ayako Sasaki for her skillful technical support. The insightful comments of two anonymous reviewers and the editor are also appreciated.
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Kusumi, J., Su, ZH. Isolation and characterization of 15 polymorphic microsatellite markers for the fig-pollinating wasp, Blastophaga nipponica (Hymenoptera: Agaonidae). Appl Entomol Zool 49, 487–491 (2014). https://doi.org/10.1007/s13355-014-0267-x
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DOI: https://doi.org/10.1007/s13355-014-0267-x